미국 Accegen은 생명과학 연구와 의약품 개발을 지원하기 위해 다양한 세포주와 생물학적 제품을 제공하는 바이오 제품 제조 기업입니다.

제품 설명

JIMT-1

제품 번호

ABC-TC504S

제품 특징

Description

JIMT-1 is a human breast cancer cell line derived from the pleural effusion of a 62-year-old female patient with ductal breast carcinoma. The cells are of epithelial progenitor cell origin and exhibit high expression of HER-2 mRNA and protein, with a HER-2 oncogene amplification. These cells are resistant to HER-2-targeting drugs and lack estrogen and progesterone receptors, making them a valuable model for studying HER-2-related drug resistance. JIMT-1 cells grow as an adherent monolayer in DMEM medium supplemented with 10% fetal bovine serum, with the ability to form xenograft tumors in mice. This cell line does not show any specific viral infections (e.g., HPV, HIV, or EBV), but its HER-2 antibody resistance is a key characteristic.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

• • • 
      

    • JIMT-1 breast cancer cell line offers crucial roles in cancer research and drug development. It can generate xenograft tumors in mice models, allowing researchers to evaluate drug efficacy and inhibitor effectiveness both in vitro and in vivo. For instance, JIMT-1 cells have been extensively used to test HER-2-targeting drugs and small molecule tyrosine kinase inhibitors designed to block the HER-2 signaling pathway. Additionally, JIMT-1 cells can be utilized for genomic screening to identify key genes involved in cell proliferation and tumor growth. These applications make JIMT-1 a valuable tool for understanding breast cancer biology, investigating mechanisms of therapeutic resistance, and developing targeted therapies.

Frequently Asked Questions

• What is the doubling time of JIMT-1 cell line?

  JIMT-1 cell line doubling time is 30-40h, and it is recommended to change medium every 2-3 days.

• What is the property of JIMT-1 cell?

  JIMT-1 cells overexpress HER-2 mRNA and are insensitive to drugs that inhibit HER-2 (such as trastuzumab). JIMT-1 cells have an amplified HER-2 oncogene, which showed no identifiable mutations in its coding sequence.

  The cell line lacks expression of hormone receptors (estrogen receptors and progesterone receptors) and is phenotypically of epithelial progenitor cell origin.

• What is the suggested cell culture concentration (cells/ml) of JIMT-1 cell line?

  The recommended cell density for JIMT-1 cells before passage is 1-2 × 10^5 cells/mL. We recommend to use a T-25 setup at a dilution of 1:5-1:10 (our cell count is approximately 1-2M/vial).

• Recommended Medium And Supplement

• 
  

• ---

  
  

Accegen의 모든 제품들을 만나 보세요!

PRODUCTS

• Primary Cells
• Tumor Cell Lines
• MicroRNA Agomir/Antagomir
• Stem Cells
• Immortalized Cell Lines
• Transfected Stable Cell Lines
• Nucleic Acid Kits

Accegen - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"