DSMZ no.:

Species:

human (Homo sapiens)

Cell type:

B cell lymphoma

Origin:

established from the peripheral blood of a 37-year-old white man with low-grade follicular small cleaved cell lymphoma (B-NHL, stage IVB) after chemotherapy and transformation to small cell non-cleaved non-Hodgkin´s lymhoma in 1989; described in the literature to carry t(14;18) and t(8;11) with BCL2 and MYC rearrangements, respectively

Reference(s):

14775, 14969, 14774

Biosafety level:

1

Permissions and restrictions:

Morphology:

round to polymorph cells growing singly in suspension; image; image; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 0.5-1.0 x 10⁶ cells/ml; maintain at 0.25-4.0 x 10⁶ cells/ml; optimal split ratio of 1:3 to 1:5 about 2-3 times a week

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 20-30 hours

Harvest:

maximum density at ca. 4.5 x 10⁶ cells/ml

Storage:

frozen in serum-free freezing medium (Bambanker^TM)

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3-, CD10+, CD13-, CD19+, CD20+, CD34-, CD37+, CD38+, CD80-, CD138-, HLA-DR+, cyCD79a+, sIgG-, sIgM+, sIg-kappa+, sIg-lambda-, cIgG-, cIgM+, cIg-kappa+, cIg-lambda-; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hyperdiploid karyotype with 4% polyploidy; 47(46-50)<2n>XY, -13, +2mar, add(1)(p34), del(6)(q25), t(8;11)(q24.3;q24), i(13q), t(14;18)(q32;q21); t(14;18) effects IGH-BCL2 rearrangement

Molec. Genetics:

expression of fusion gene IGH-BCL2 (MBR) confirmed by RT-PCR

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as: