DSMZ no.:

Species:

human (Homo sapiens)

Cell type:

B cell lymphoma

Origin:

established in 1983 from the ascites of a 52-year-old man with B-NHL (diffuse, undifferentiated, small non-cleaved large cell)

Reference(s):

Biosafety level:

1

Permissions and restrictions:

Morphology:

lymphoid cells growing singly or in clumps in suspension; image; image; image

Medium:

80% RPMI 1640 + 20% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/ml; maintain at 0.7-1.5 x 10⁶ cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 5-8 days

Harvest:

maximum density at ca. 1.8 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hyperdiploid karyotype with 16% polyploidy; 49(45-51)<2n>XY, +7, +17, +21, der(3)inv(3)(p24q24-q25)t(3;13)(q24-q25;q32), add(5)(p11), der(13)t(3;13)(q24-q25;q32), t(14;18)(q32;q21), der(17)dup(17)(p12p13)t(17;20)(q11.2;q11.2), r(17), add(19)(q12), der(19)t(19;20)(p13;p11), der(20)t(17;20)(q11.2;q11.2)x1-2; carries t(14;18) effecting rearrangement of BCL2 with IGH (IGH); resembles published karyotype

Molec. Genetics:

expression of fusion gene IGH-BCL2 confirmed by RT-PCR

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

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