DSMZ no.:

Species:

human (Homo sapiens)

Cell type:

urinary bladder carcinoma

Origin:

established from the primary bladder carcinoma of a 68-year-old man in 1974; cells are known to produce several growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.)

Reference(s):

15203, 14539, 15057, 14643, 14867

Biosafety level:

1

Permissions and restrictions:

Morphology:

epithelial-like adherent cells; image; image; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 1-2 x 10⁶ cells/80 cm²; split confluent cultures 1:4 to 1:5 using trypsin/EDTA (cells should be incubated for 5 to 10 min with trypsin/EDTA at 37 °C)

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 24 hours

Harvest:

about 8-10 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Mycoplasma:

original contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypotriploid karyotype with 17% polyploidy - 62(47-64)<3n>XX, -Y, -4, -5, -10, -13, -13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?), del(6)(q23), der(9)t(9;?)(p12;?), der(11)t(5;11)(q14;p12), ?der(12)t(12;?)(q14;?), i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13) - carries large submetacentric marker with q arm resembling tandem dup(7) - 9p12 is a recurrent breakpoint in bladder carcinoma

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

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