DSMZ no.:

Species:

human (Homo sapiens)

Cell type:

esophageal adenocarcinoma

Origin:

established in 1993 from the primary tumor of a 72-year-old man with esophageal adenocarcinoma; cell line also known as JROECL19

Reference(s):

16110, 16055, 18232

Biosafety level:

1

Permissions and restrictions:

Morphology:

epithelial cells growing adherently as monolayer; image; image; image

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS

Subculture:

seed out at ca. 2 x 10⁶ cells/80 cm² flask; split confluent culture 1:5 every 2-3 days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 50-60 hours

Harvest:

cell harvest of ca. 10 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded hypotriploid karyotype with 4% polyploidy; 58-62<3n>XX, -Y, +1, -4, -5, +8, -10, -14, -14, -15, -15, -17, -18, -18, -21, -21, -22, -22, +6mar; del(X)(p11)ins(X;?)(q11?), add(1)(p11), del(2)(q12), add(3)(p21), add(4)(q11), del(p14), der(4)t(4;4)(p11;q24), del(5)(q11q21), der(6)add(6)(p11)add(6)(q25), add(6)(q11), i(6)(p10), del(7)(q12), add(9(p21), add(10)(p11), del(10)(p12), i(11)(p10), add(12)(p11), i(12)(p10), der(12;13)(p10;q10), add(13)(q32), inv(15)(p11q21), der(16)t(1;16)(q24;p13), add(18)(q23)

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -

Supplied as: