본문 바로가기 주메뉴 바로가기

제품소개

제품상세페이지

BioinGentech

[BioinGentech] One-Step qPCR-realtime™ SARS CoV-2 E-ROX and ORF1ab-FAM and S-CY5 genes

Cat-No. SKU: S831


BioinGentech는 유전자 기술과 생명과학 분야에서 혁신적인 솔루션을 제공하는 선도적인 회사입니다.




제품 설명


One-Step qPCR-realtime™ SARS CoV-2 E-ROX and ORF1ab-FAM and S-CY5 genes




제품 번호


SKU: S831

 



제품 설명


Multiplex OneqPCR- Raltime TM SARS CoV-2 (E, ORF1ab and S genes) amplifies  and detect the E gene (SARS CoV-2), ORF1ab gene (SARS CoV-2),  S gene (SARS CoV-2) and Rp gene (Rnase P human) in Single One Step RT-PCR Reaction.

The kit contains Primer & Probe Mix for the detection of E gene (CY5), ORF1ab gene (FAM), S genes (CY5) and Rp gene (HEX)

Positive Control for the amplification of the E gene, ORF1ab and S gene is also included.


Description

The One qPCR Enzyme Mix includes Reverse Transcriptase, Recombinant Ribonuclease Inhibitor in an optimized formulation, also include Bioingentech® Taq DNA polymerase and all reagents for an optimized RT-qPCR. The SARS CoV-2 (E-ORF1ab-S gene) primer and probe mix provided in the kit hybridize in specific and conserved zones of two genes. The first one is E gene which generate an envelope protein which plays a central role in virus morphogenesis and assembly, ORF1ab which generates a replicase polyprotein involved in the virus transcription and replication, on the other hand, S gene generate a Spyke glycoprotein on the virion surface which mediates receptor recognition and membrane fusion.

These genes can be detected through your real time platform by the 5’ nuclease PCR detection method. During PCR amplification, forward and reverse primers hybridize to the SARS CoV-2 target cDNA generated. Fluorogenic probes are included in the same reaction mixture. E gene is amplified and detected in ROX channel; ORF1ab gene is amplified and detected in FAM channel and S gene is amplified and detected in CY5.

An additional primer/probe set to detect the human RNase P gene (RP) as internal control to monitor PCR inhibition is also included in the panel labeled with a 5-reporter and a 3-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. As a result, a fluorescence increase can be detected on a range of real time PCR platforms through HEX/VIC channel.

Figure 1: Clinical tests of the One Step qPCR-realtime™ SARS CoV-2 E-ROX and ORF1ab-FAM and S-CY5 genes detection Kit were performed with more than 5000 samples from asymptomatic and symptomatic patients.
Total RNA was isolated followed by a One Step RT-PCR for the detection of the SARS CoV-2 E gene, SARS CoV-2 ORF1ab, SARS CoV-2 S gene and the human RNase P gene.

 



BioinGentech의 모든 제품을 만나 보세요!


Human Pathogen Detection

Veterinary Pathogen Detection

Mycoplasma Pathogen Detection

Bioterios Pathogen Detection

Agroindustrial Pathogen Detection

Water Pathogen Detection

SARS-CoV-2 PCR KIT / 1 Gen

SARS-CoV-2 PCR KIT / 2 Genes

SARS-CoV-2 PCR KIT / 3 Genes



BioinGentech - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"