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제품 설명 

LentiPlus Pseudovirus Assembly and Infection Kit

제품 번호

옵션 선택

제품 특징

For Highly Efficiency Lentiviral Particle Assembly & Infection

The LentiPlus Pseudovirus Assembly and Infection Kit provide a convenient and efficient tool for producing lentiviral particles to deliver and express your gene of interest in dividing, non-dividing, or primary cells. The multi-vector pre-mixed Packaging Mix is easy to use and designed to express viral structural proteins and an envelope protein of your choice at ratios optimal for lentiviral assembly and production. Choose from over 14 viral envelopes to further optimize transduction to your target cell type. In addition, the Kit also provides a lentiviral transduction enhancer, Infectin^TM, a proprietary technology developed at Virongy, to significantly increase the productive viral transduction rate (up to 50-fold). Infectin^TM paired with the VSV-G (vesicular stomatitis virus glycoprotein) envelope protein for transduction efficiencies as high as 99% in certain cell types. The use of Infectin^TM eliminates the need to concentrate viral particles, and greatly facilitates biological and biochemical characterization of lentiviral transduced cells.

Highlights

• Pre-opitimized vector mix, ready to use, and easy steps
• Higher yield
• Infectin^TM technology that enhances transduction efficiency up to 30 fold
• No need for viral particle concentration
• High transduction efficiency for both cell lines and primary cells.

Kit Components of the LentiPlus Pseudovirus Assembly and Infection Kit

(with Tranfectin and Lenti-Infectin^TM reagents)

Example Assembly Protocol:

Lentiviral particle assembly using Lenti2go Lentiviral Kit (10 cm dish)

1. One day (18-24 hours) prior to co-transfection, plate 4 x 10⁶ HEK293T cells in a 10 cm dish, and grow at 37°C to reach about 80% confluence.
2. Remove medium from the dish and rinse with serum free DMEM medium, then add 9 ml of warm, serum-free medium.
3. Prepare transfection solution: Set up two tubes (A) and (B). (A) is for DNA dilution, and (B) is for dilution of Transfectin.
4. In (A), add 10 μg of LentiPlus Master Mix, add 10 µg of Lenti-viral vector of your choice, and then add serum-free DMEM medium to make a final volume of 500 μl, Mix well.
5. In (B), add 45 μl of Transfectin, and then add 455 μl if serum-free DMEM medium to make a final volume of 500 μl. Mix well.
6. Combine (A) and (B) into a total volume of 1 ml. Mix DNA and Transfectin by votexing, and incubate at room temperature for 15 min.
7. Add the 1 ml (A) + (B) mixture to the dish drop-wise. Mix gently by slowly rotating the dish. Incubate at 37°C for 6 hours.
8. Carefully remove the supernatant with a pipette (not to remove cells). Add 10 ml of warm DMEM with serum. Culture cells for 48 hours
9. Harvesting virus at 48 hours. Filter through a 0.45 μm filter to remove cellular debris. Aliquot and store the virus at -80°C.

LentiPlus Vector Assembly & Infection Kit

고효율 Lentiviral Particle Assembly & Infection을 위한 솔루션

LentiPlus Pseudovirus Assembly and Infection Kit는 분열 세포(dividing cells), 비분열 세포(non-dividing cells), 그리고 primary cells에서 관심 유전자를 효율적으로 전달하고 발현할 수 있도록 lentiviral particle을 손쉽게 생산할 수 있는 키트입니다.

미리 최적화된 Multi-vector Packaging Mix를 제공하여 사용이 간편하며, Lentiviral particle의 assembly 및 생산에 최적화된 비율로 viral structural proteins와 원하는 envelope protein을 발현할 수 있도록 설계되었습니다. 또한 14종 이상의 viral envelope 옵션을 제공하여 목표 세포에 맞는 transduction 효율을 더욱 향상시킬 수 있습니다.

키트에는 Virongy의 독자 기술인 Turbo-Infectin™이 포함되어 있어 productive viral transduction efficiency를 최대 30배까지 향상시킬 수 있습니다. 특히 VSV-G(Vesicular Stomatitis Virus Glycoprotein) envelope protein과 함께 사용할 경우 일부 primary cell에서는 최대 99%의 transduction efficiency를 제공합니다.

Turbo-Infectin™을 사용하면 별도의 viral particle 농축 과정 없이도 높은 transduction 효율을 얻을 수 있어, Lentivirus가 도입된 세포의 생물학적 및 생화학적 분석을 더욱 간편하게 수행할 수 있습니다.

주요 특징 (Highlights)

• 
  
• 사전 최적화된 Vector Mix 제공으로 즉시 사용 가능
• 간편한 실험 프로토콜
• 높은 Lentiviral particle 생산 수율
• Turbo-Infectin™ 기술로 최대 30배 향상된 Transduction Efficiency
• Viral particle 농축 과정 불필요
• Cell line 및 Primary cell 모두에서 우수한 Transduction Efficiency 제공
• 
  

Applications

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• Target cell의 Lentivirus 및 Retrovirus Transduction 효율 향상
• 다양한 Envelope Virus의 Infection Rate 향상
• Cell 또는 Tissue Culture에서 Infectious Virus Recovery 향상
• Antiviral Drug Screening 효율 향상 

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