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제품 설명

MiQuant® CAR-T Cell Lenti – dPCR

제품 번호

58-0701 (35ul primer/probe mix)

58-0702 (140ul primer/probe mix)

제품 특징

The MiQuant® CAR-T Cell Lenti – dPCR kit is intended for the determination of vector copy numbers (VCN) in transduced cells to determine the average copies of integrated lentiviral vectors per cell genome after ex vivo transduction via digital PCR (dPCR). This kit provides a specific primer/probe mix and offers an open-platform solution for use with various dPCR devices (QIAcuity®, ddPCR™, Quantstudio™ Absolute Q™). MiQuant® CAR-T Cell Lenti – dPCR works for all HIV-1 based lentivirus transduction systems.

Type of PCR

Digital PCR (dPCR)

Recommended Use

MiQuant® CAR-T Cell Lenti – dPCR can be used for the characterization of cellular products and for in-process control as well as final QC release testing in cell therapy production processes.
Besides these applications the MiQuant® CAR-T Cell Lenti – dPCR kit can also be used for determining VCN in cell culture samples in research and development and is applicable for human or other mammalian cells (such as mice and rats).

Kit Components

• MiQuant® Lenti Mix (lyophilized)
• Positive Control DNA (lyophilized)
• dPCR grade Water

Package Size

Cat. No. 58-0701: 35 µl primer/probe mix

Cat. No. 58-0702: 140 µl primer/probe mix

Since every dPCR device requires different PCR reaction volumes, the number of PCR reactions are shown in the following table.

Result Evaluation

Absolute Quantification

Required Lab Device and Consumables

• Digital PCR device with corresponding consumables (plates or stripes)
  • QIAcuity®
  • Quantstudio™ Absolute Q™
  • QX600™ or QX200™ ddPCR™
• Corresponding Mastermix (QIAcuity® Probe PCR Kit (4x), Absolute Q™ DNA Digital PCR Master Mix (5x), ddPCR™ Multiplex Supermix (4x) or ddPCR™ Supermix for Probes (2x))
• MiQuant® SP Beads (sample preparation kit, Cat. No. 56-4010/-4100)
• 1.5 ml reaction tubes (low-bind, DNA- and RNA-free)
• Microcentrifuge for 1.5 ml PCR tubes
• Vortex mixer
• Pipettes with corresponding filter tips

Optional:

• Restriction enzymes (EcoRI-HF and NruI-HF; 20 U/μl)
• PCR Clean™ (Cat. No. 15-2025 and 15-2001)

Shelf Life and Storage

Shipment at ambient temperature. The expiry date of the unopened package is marked on the package label. The kit components must be stored at +2 to +8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month.

Calculate the Vector Copy Number with our tool.
Data Analysis Application

Minerva Biolabs offers a free sample of this product for testing:
Request free sample

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Cell Culture Contamination Control

DNA / RNA Extraction

PCR Reagents & Enzymes

Pharma / ATMP

Decontamination Reagents for PCR Labs

Water Contamination Testing

Proficiency Testing Program 2024

Contract Manufacturing

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