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제품 설명 

Anti-Tocilizumab ELISA Kit

제품 번호

KAC36901 

제품 특징

Catalog No.

KAC36901

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Tocilizumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Tocilizumab will be captured by immobilized Tocilizumab. After washing away any unbound substances, a biotin-labeled Tocilizumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Tocilizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

1.56 - 100 ng/mL

Sensitivity

0.81 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

Atlizumab, MRA / R-1569 / RHPM-1 / RO-4877533,CAS: 375823-41-9

Data Image

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  Experiment Example
  CALCULATION OF RESULTS
  Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Anti-Tocilizumab concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

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