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제품 설명
제품 번호
Cat. E-EL-H6156
제품 특징
●Sensitivity: 0.94 pg/mL.
●Detection Range: 1.56-100 pg/mL
●Specificity: This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.
●Repeatability: Coefficient of variation is < 10%
Sample collection
Serum: Allow samples to clot for 1 hour at room temperature or overnight at 2-8℃before centrifugation for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay.
Plasma: Collect plasma using EDTA-Na2 as an anticoagulant. Centrifuge samples for 15 min at 1000×g at 2-8℃ within 30 min of collection. Collect the supernatant to carry out the assay.
Cell lysates: For adherent cells, gently wash the cells with moderate amount of pre-cooled PBS and dissociate the cells using trypsin. Collect the cell suspension into a centrifuge tube and centrifuge for 5 min at 1000×g. Discard the medium and wash the cells 3 times with pre-cooled PBS. For each 1×106 cells, add 150-250 μL of pre-cooled PBS to keep the cells suspended. Repeat the freeze-thaw process several times or use an ultrasonic cell disrupter until the cells are fully lysed. Centrifuge for 10 min at 1500×g at 2-8℃. Remove the cell fragments, collect the supernatant to carry out the assay.
Tissue homogenates: It is recommended to get detailed references from the literature before analyzing different tissue types. For general information, hemolyzed blood may affect the results, so the tissues should be minced into small pieces and rinsed in ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then homogenized in PBS (tissue weight (g): PBS (mL) volume=1:9) with a glass homogenizer on ice. To further break down the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifuged for 5-10 min at 5000×g at 2-8℃ to get the supernatant.
Cell culture supernatant or other biological fluids: Centrifuge samples for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay.
Recommended reagents for sample preparation: 10×EDTA Anticoagulant (Cat No. E EL-SR003), PMSF Protease Inhibitor (Cat No. E-EL-SR002), 0.25% Trypsin Solution (Cat No. EP-CM-L0043).
Assay Procedure Summary
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