• Exceptional fidelity, approximately 50 times higher than Taq polymerase
• Amplifies targets up to 5–10 kb in size
• Produces blunt-end PCR products
• Efficient amplification of both low- and high-complexity targets
• High resistance to inhibitors for robust performance across a variety of sample types

• High-fidelity PCR
• Generation of PCR products for blunt cloning

biotechrabbit™ ExactiFi™ Hot-Start DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. ExactiFi exhibits approximately 50 times higher accuracy than Taq DNA polymerase and amplifies targets up to 5–10 kb in size.

The enzyme catalyzes template-dependent nucleotide polymerization in the 5'→3' direction. Additionally, the 3'→5' exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5'→3' exonuclease activity and no detectable reverse transcriptase activity, producing blunt-end PCR products.