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제품 설명
1× dsDNA HS Assay Kit
제품 번호
12642ES
제품 설명
1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.
Features
Components No. | Name | Concentration | 12642ES60 (100 T) | 12642ES76 (500 T) |
12642-A | 1×dsDNA HS Working Solution | 1×Concentrate in DMSO | 50 mL | 250 mL |
12642-B | dsDNA Standard 1 | 0 ng/μL in TE buffer | 1 mL | 5×1 mL |
12642-C | dsDNA Standard 2 | 10 ng/μL in TE buffer | 1 mL | 5×1 mL |
This product should be stored at 2~8℃ away from light for 12 months.
dsDNA quantification; DNA concentration determination
1. Easy-to-use and streamlined quantification workflow
Figure 1. DNA Quantification Workflow
2. Good linearity: 12642 has good linearity in the range of 0.2-100 ng.
Figure 2. Linear Relationship Test
Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12642. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA.
3. Dye binding specificity
Figure 3. Specificity test
Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested.
Conclusion: Adding equal amounts of ssDNA slightly increases the 12642 reading; error <10%(left). Adding equal amounts of RNA also increases the 12642 reading; error <10%(right).
4. Efficient dye binding and stable fluorescence signal
Figure 4. Dye binding efficiency and fluorescence signal persistence
Test Scheme: Two RNA libraries of different concentrations were measured using 12642 on the Qubit 3.0 with a 2 μL sample volume.
Conclusion: The fluorescence signal saturated within 2 minutes and remained stable for up to 5 h.
5. Strong tolerance to common contaminants
6. Ultra-high stability
Figure 5. Stability comparison of Yeasen's (Cat#12642ES) and competitor T brand
Test Scheme: Two dsDNA concentrations were measured over time using supplier T* (4°C), Yeasen#12642 (4°C), and Yeasen#12642 (room temperature, ~25°C). Deviation from the initial value (Day 0) was calculated.
Conclusion: Yeasen#12642 remained stable at room temperature for 2 weeks, with deviations <10%.
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