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제품 설명
Hifair™ miRNA 1st Strand cDNA Synthesis Kit (poly A)
제품 번호
11148ES10 / size: 10T
11148ES50 / size: 50T
제품 설명
MicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals. This kit uses Poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The 2×Hifair™ miRNA RT buffer in the product contains all the raw materials and primers for miRNA Poly(A)-tailing reaction and reverse transcription reaction. Careful optimization ensures that Poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end.
This product is recommended to be used in conjunction with our company's Hieff™ miRNA Universal qPCR SYBR Master Mix (Cat#11171) for optimal experimental results.
| Final product type | cDNA (First-Strand) |
| PCR method | RT-PCR |
| Reaction format | Isolated fraction |
| Sample type | miRNA |
| Optimal reaction temperature | 37℃ |
| Reverse transcriptase | MMLV |
| Components No. | Name | 11148ES50 (50T) |
| 11148-A | Hifair™ miRNA RT enzyme mix | 87.5 μL |
| 11148-B | 2×Hifair™ miRNA RT buffer | 250 μL |
| 11148-C | RNase-free H2O | 10 mL |
| 11148-D | Universal Reverse Primer (10 μmol/L) | 4 mL |
| 11148-E | U6 Forward Primer (10 μmol/L) | 500 μL |
| 11148-F | U6 Reverse Primer (10 μmol/L) | 500 μL |
The product should be stored at -25°C~-15℃ for 1 year.
Figure1. High sensitivity detection rate: 10 pg RNA could be detected.
The synthesized hsa-miR-let-7e-5p (a) and 293T cell total RNA (b) were used as templates and diluted to the following gradients: 60 copies to 606 copies (6 gradients) and 10 pg-100 ng (5 gradients). All gradient cDNA was detected.
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