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제품 설명
Mouse Tissue Direct PCR Kit (With Dye)
제품 번호
10185ES50 / size: 50T
10185ES50 / size: 200T
제품 설명
This kit can directly and quickly conduct PCR amplification of mouse tissue (such as mouse tail, mouse ear, mouse toe, muscle, etc.), and has strong sample compatibility. Equipped with a powerful lysis buffer, this kit can rapidly lyse samples and release genomic DNA. The lysate can be directly added to the PCR reaction system without purification, and the operation is convenient. In addition, this kit requires low sample input, and 5 mg mouse tissue or 1-5 mm mouse tail can be used for experiments.
The 2× Mouse Direct PCR Mix provided by this kit is a hot-start PCR reaction solution with a 2-fold concentration. It contains all the components used for PCR amplification except the template and primers, which greatly simplifies the operation process and reduces the chance of contamination. The kit can be used for transgene identification, mouse genotyping, etc.
Features
Applications
Product specification | Kit |
Hot start | Built-in Hot Start |
Conditions for transportation | Ice Packs |
Product type | Direct PCR Kit |
Apply to (application) | Mouse tail, Mouse ear, Rat toe, Viscera, Skin, etc. |
Components No. | Name | 10185ES50 | 10185ES70 |
10185-A | Buffer ML | 5×1 mL | 20×1 mL |
10185-B | Buffer MT | 0.6 mL | 2×1.25 mL |
10185-C | 2× Mouse Direct PCR Mix | 500 μL | 2×1 mL |
a) Buffer ML is a lysis buffer containing strong protein denaturants, please wear gloves.
b) Buffer MT is a stop buffer used to stop the lysis function of Buffer ML.
c) 2× Mouse Direct PCR Mix: Contains hot-start Taq DNA polymerase, dNTP mix, MgCl2 , reaction buffer, PCR reaction enhancer, optimizer, stabilizer, electrophoresis indicator dye, etc.
1. Component A: The product should be stored at 2°C~8°C for one year. For multiple use for a long time, please avoid cross-contamination.
2. Component B/C: The product should be stored at -25℃ ~ -15℃ for one year. Please avoid repeated freeze-thaw.
1. Target gene (within 1 kb) amplification result
Figure 1. Suitable for target gene amplification within 1 kb.
2. The extension speed demostration
Figure 2. 500bp gene, the extension speed can be as fast as 1sec/kb.
Cited from "TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell . 2022 Mar 17;185(6):980-994.e15. doi: 10.1016/j.cell.2022.02.010. "
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