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제품 설명
Hieff Canace™ Veritas Long PCR Master Mix (With Dye)
제품 번호
10166ES
제품 설명
Hieff Canace™ Long PCR Master Mix (With Dye) is a ready-to-use 2× pre-mixed solution containing Hieff Canace™ High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system, which contains pre-added electrophoresis indicators. The pre-mix contains pre-added electrophoresis indicator, PCR products can be directly electrophoresed, the amplification products are flat ends. 2× Hieff Canace™ Long PCR Master Mix (With Dye) has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with only the primers and templates. In addition, the product also contains a specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.
Ultra-high fidelity: Low mismatch rate in mutation-prone high GC gene;
Fast speed: The speed is as fast as 5 sec/kb;
Wide applicability: It can be used to amplify genes with 20-80% GC content, and tolerate 20% blood and mouse lysates;
Good stability: Stored at 37°C for 7 days without affecting performance;
Easy use: Just adding primers and templates for amplification, with blue loading dye for direct electrophoresis.
Faster – Bacterial plasmids up to 3 kb can be processed in as little as 1 second per kb.
More Versatile – Efficiently handles GC content up to 70% and fragment lengths up to 10 kb.
More Reliable – Validated on hundreds of fragments across over ten species.
Higher Efficiency – High specificity with detection rates up to 100%.
More Stable – Maintains stability after 100 freeze–thaw cycles, with a shelf life of up to two years.
| Cat.No. | 10166ES01 | 10166ES03 | 10166ES08 |
| Size | 250 μL | 1 mL | 5×1 mL |
This product should be stored at -25~-15℃ for 1 years.
Figure1. High sensitivity of Hieff Canace™ High-Fidelity Long PCR Master Mix.
Figure 2. Long PCR from cDNA by Hieff Canace™ High-Fidelity Long PCR Master Mix.
Components | Volume（μL） | Final concentration |
Hieff Canace™ Long PCR Master Mix (With Dye)* | 25 | 1× |
Template** | x | - |
Forward Primer（10 μmol/L）*** | 2 | 0.4 μmol/L |
Reverse Primer（10 μmol/L） | 2 | 0.4 μmol/L |
ddH2O | Up to 50 | - |
Table 1 PCR reaction system
*In 1× premixes containing 2 mM Mg2+ and 200 μM dNTPs.
**Recommended range 10-200 ng, cDNA sample upload volume range not more than 1/10 of the reaction system, recommended 1-2.5 μL.
***The final primer concentration in the PCR reaction system ranges from 0.2-1 μM, and 0.4 μM is recommended.
Cycle step | Temp. | Time | Cycles |
Initial denaturation | 98℃ | 30 sec | 1 |
Denaturation | 98℃ | 10 sec | 30-35 |
Annealing* | 60℃ | 5 sec | |
Extension** | 72℃ | 5-10 sec/kb | |
Final extension | 72℃ | 2 min | 1 |
Table 2 PCR reaction program
*Recommended temperature: 60°C, a temperature gradient can be set up to find the optimal temperature for primer annealing. The recommended annealing time is set to 5 sec and can be adjusted from 5-30 sec. Too long annealing time may result in diffuse amplification products on the gel.
**Extension time: Recommended 5 sec/kb, can also be extended to 10 sec/kb as needed.
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