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제품 설명
Mouse Cochlear Hair Cell Line (HEI-OC1)
제품 번호
YC-A025
제품 설명
3. STR Authentication
| Loci | STR Info (Sample Cell) Sample Cell Line: HEI-OC1 | STR Info (Cell bank) Cell Line: HEI-OC1 | |||||
| Allele1 | Allele2 | Allele3 | Allele4 | Allele1 | Allele2 | Allele3 | |
| 18-3 | 15 | 16 | 15 | 16 | |||
| 4-2 | 19.3 | 19.3 | |||||
| 6-7 | 17 | 17 | |||||
| 19-2 | 12 | 13 | |||||
| 1-2 | 17 | ||||||
| 7-1 | 26.2 | ||||||
| 8-1 | 16 | ||||||
| 1-1 | 17 | ||||||
| 3-2 | 14 | ||||||
| 2-1 | 16 | ||||||
| 15-3 | 26.3 | 26.3 | |||||
| 6-4 | 17.3 | 17.3 | |||||
| 13-1 | 16.1 | 17.1 | |||||
| 11-2 | 15.2 | ||||||
| 17-2 | 14 | ||||||
| 12-1 | 16 | 16 | |||||
| 5-5 | 13 | 13 | |||||
| X-1 | 25 | 25 | |||||
| TH01 | |||||||
| D5S818 | |||||||
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
Cryopreserved cells: In the case of cryopreserved cells transported with dry ice, upon received, immediately transfer toliquidnitrogen for storage or store briefly at -80°C freezer, or proceed directly to cell thawing. Uponcell thawing, please count the cell number and cell viability and take some photos of the cells under different magnification (e.g. at 100x and 40x) as the records.
Upon received, please ensure to takes photos of the package, including dry ice andthetubes, and contact us within 24 hrs if any abnormalities such as dry ice has ran out, the cap of thecryovial isdislodged, broken and the cell is contaminated.
1) Preparation: warm up the complete culture medium in 37°C water bath for 30 mins. Transfer the cryopreserved vial from liquid nitrogen to - 80°C freezer, and leave for several minutes to volatilize residual liquid nitrogen; 2) Inside the ultra-clean bench, pipet 6-7 mL of complete medium into a 15 mL centrifuge tube; 3) Take out the cryopreserved vial from - 80℃ freezer and leave in dry ice temporarily, shake slightly before thawing to remove residual dry ice and liquid nitrogen. Then hold the cap with forceps, quickly thaw cells in a 37°C water bath by gently swirling the vial (Note: keep the cap out of the water). In about 1 minute, it would completely thaw; 4) Inside the ultra-clean bench, sterilize the outer surface of the vial by wiping with an alcohol cotton pellet and leave it to dry. Transfer the thawed cells to the prepared centrifuge tube (step 2) by pipette, close the lid, and centrifuge at 1100 rpm for 4 mins at room temp to collect the cells; 5) Inside the ultra-clean bench, carefully remove and discard the supernatant. Resuspend cell pellet with 1mL of fresh complete medium and then transfer to a T25 flask (or 6 cm culture dish) containing 4 mL of complete medium, label the flask with cell name, date and passage no., incubate the flask in a 37℃, 5%CO2 incubator.
Note: Please do not thaw the cells directly to a T75 flask or 10 cm culture dish.
1) As long as the cells are 80%-90% confluent, it is ready to passage. Inside the ultra-clean bench, remove and discard the medium from the flask and briefly rinse the cell 1-2 times with 1×PBS (2-3 mL for T25 flask, 4-5 mL for T75) to remove residual medium and serum; 2) Add the corresponding volume of trypsin solution (See Table 1 of the instruction manual for details) and allow trypsin completely cover the cells, place the flask into the incubator and incubate for 1-2 mins (If cells are hard to digest, allow appropriate extension of incubation), until the majority of the cells become round and non-adherent as observed under the microscope, a large number of cells detached from each side when gently shaking and tapping the flask, terminate trypsin digestion immediately; 3) Add complete medium to stop digestion, the volume is 2 times of trypsin. Then gently pipet the cells several times to allow all cells to be completely detached from the flask; 4) Transfer the cell suspension with a 10 mL pipette into a 50 mL centrifuge tube, rinse the residual cells from the flask using appropriate volume of PBS , then collect and put them together to the centrifuge tube; 5) Centrifuge at 1100 rpm for 4 mins at room temp. After centrifugation, remove and discard the supernatant and resuspend the cells with 2 mL of complete medium; 6) Cells need to be passaged at appropriate passage ratio, 1:3 for the first passage, increasing the passaging ratio if the cells are grown to confluence within two days, or decreasing the passaging ratio if the cells are not grown to confluence in 3-4 days.
1) Same as procedures of cell passaging, inside the ultra-clean bench, digest the cells to a single-cell suspension, and terminate digestion by adding complete medium. All liquid is transferred to a 50 mL centrifuge tube; 2) Mix well by pipetting and take 20 μL for cell counting; 3) Centrifuge at 1100 rpm for 4 mins at room temp. After centrifugation, remove and discard the supernatant, and resuspend the cells with 1-2 mL of 4℃ pre-cooled cryopreservation medium (use the one you usually use in lab, or any commercial cryopreservation solutions are fine), then add cryopreservation medium to adjust to the required density (1x10⁶-1x10⁷cells/mL); 4) Aliquot the cell suspension to cryovials as 1 mL/tube, close the lid tightly, and the cryovials should be labeled with the cell name, source, cell passage number, and date of cryopreservation in advance; 5) Place the cryovials in 4°C pre-cooled Freezing Container, then put the container in -80℃ freezers within 15 mins after cell cryopreservation; 6) Stay overnight, transfer the cryovials to liquid nitrogen for long-term storage.
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