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제품 설명
GBA1 Knockout cell line (HeLa)
제품 번호
YKO-XN88740
제품 설명
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Detailed Product Information
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 3 (based on transcript GBA1-202)
Validation: PCR+Sanger Sequencing
STR Profile
| Loci | STR Info (Sample Cell)
Sample Cell Line:HeLa | STR Info (Cell bank)
Cell Line:HELA |
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 |
| D5S818 | 11 | 12 |
| 11 | 12 |
|
| D13S317 | 12 | 13.3 |
| 12 | 13.3 |
|
| D7S820 | 8 | 12 |
| 8 | 12 |
|
| D16S539 | 9 | 10 |
| 9 | 10 |
|
| VWA | 16 | 18 |
| 16 | 18 |
|
| TH01 | 7 | 7 |
| 7 | 7 |
|
| AMEL | X | X |
| X | X |
|
| TPOX | 8 | 12 |
| 8 | 12 |
|
| CSF1PO | 9 | 10 |
| 9 | 10 |
|
| D12S391 | 20 | 24 | 25.0 |
|
|
|
| FGA | 21 | 21 |
|
|
|
|
| D2S1338 | 17 | 17 |
|
|
|
|
| D21S11 | 27 | 28 |
|
|
|
|
| D18S51 | 16 | 16 |
|
|
|
|
| D8S1179 | 12 | 13 |
|
|
|
|
| D3S1358 | 15 | 18 |
|
|
|
|
| D6S1043 | 18 | 19 |
|
|
|
|
| PENTAE | 7 | 17 |
|
|
|
|
| D19S433 | 13 | 14 |
|
|
|
|
| PENTAD | 8 | 15 |
|
|
|
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| D1S1656 | 12 | 15 |
|
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|
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Instruction
Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GBA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GBA1 gene knockout GBA1 Knockout cell line (HeLa) will be expanded and cryopreserved.
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EZ-editor™ Culture Medium
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