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제품 설명
GBA1 Knockout cell line (Hep G2)
제품 번호
YKO-HT1595
제품 설명
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Detailed Product Information
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 3 (based on transcript GBA1-202)
Validation: PCR+Sanger Sequencing
Instruction
Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GBA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GBA1 gene knockout GBA1 Knockout cell line (Hep G2) will be expanded and cryopreserved. 
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Gene Editing Services
Stable cell Lines
Cloning Services
Virus Pakaging
CRISPR Library
Microbe
Cell Assay
Products
Red Cotton™ gRNA Plasmid Bank
EZ-editor™ Cell Line
IVD positive controls
EZ-editor™ Culture Medium
EZ-editor™ Kits
Knockout-cell-lysate
Lentivirus
UBIGENE - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"
MSDS
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