Synbio Technologies는 혁신적인 합성 생물학 솔루션을 제공하여 생명과학 연구 및 개발을 지원하는 기업입니다.
The General Introduction of Oligo Libraries
Synbio Technologies uses high-throughput semiconductor chip synthesis technology to provide customers with the design and synthesis of various library oligos. This includes oligo pools, trimer oligos, and sgRNA library oligos. Each chip can synthesize up to nearly one million oligos, reducing the research, production time, and especially cost. These oligos are widely used in NGS capture probe libraries, mutant libraries, CRISPR sgRNA libraries, etc.
Highlights
High Throughput
Up to 1 million oligos per chip
Fast Turnaround Time
Delivery time can be as fast as one week
Cost-Effective
Competitive prices and affordable services
Technical Support
Professional experts provide professional technical support
Service Details
Oligo Pools
Synbio Technologies’ high throughput synthesis platform utilizes inkjet technology to synthesize millions of oligonucleotide sequences on a chip in a single parallel run (up to 300 nt.) During the synthesis process, there is zero cross-contamination and the synthesized sequences are highly accurate and homogeneous. The customized synthesis service will perfectly match customers' downstream experiments and applications.
| Length/nt | <10,000 Oligos | 10,000-23,232 Oligos | 23,233-46,465 Oligos | 46,465-92,928 Oligos | >92,929 Oligos |
|---|---|---|---|---|---|
| <120 | 2-3 Weeks | 3-4 Weeks | 3-4 Weeks | 3-4 Weeks | 3-4 Weeks |
| 120-150 | 3-4 Weeks | 3-4 Weeks | 3-4 Weeks | 3-4 Weeks | Inquire |
| 150-200 | 4-5 Weeks | 3-4 Weeks | 4-5 Weeks | 4-5 Weeks | Inquire |
With synthesized oligo pools, our customers can then streamline various downstream applications within their research. These downstream applications include:
● Generating native DNA sequences
● Creating de novo DNA sequences
● Building genes, chassis, operons, pathways, and genomes
● Building DNA variant libraries
● Improving the features of a protein
● Testing all orthologs of a gene
● Optimizing any antibody through affinity maturation
Trimer Oligos
Synbio Technologies can insert up to 20 Trimer sites in a single Trimer oligo to ensure the diversity and convenience of downstream screening. We provide a variety of amino acid customization services according to the actual needs of customers. Trimer oligo synthesis can be used for downstream library construction, used widely in protein directed evolution, antibody screening, drug target screening/drug discovery, enzyme optimization. and other research.
| Length | Number of trimer sites in one oligo | Purification | Turnaround TIme | Price |
|---|---|---|---|---|
| ≤120 nt | ≤20 | PAGE / HPLC | 2-6 weeks | Quote |
Synbio Technologies delivers highly customized trimer libraries with amino acid types and proportions that meet customer requirements.
Take the sequence below as an example, the X represents the codon position where the mutation is required.
ACCG*************TGCCX1X2X3X4X5X6TGTX7X8X9X10X11X12X13X14X15X16X17TACX18TACT*************ACTT
X1 Trimer=A(80%):G(3%):R(3%):S(3%):T(8%):V(3%)
Table 1 The ratio of the synthesized amino acids with the actual requirements (Only partial amino acids are shown)
| Amino Acid Abbreviation | Target Value | Actual Value | Target Value | Actual Value | Target Value | Actual Value | Target Value | Actual Value | …… |
|---|---|---|---|---|---|---|---|---|---|
| Site (%) | X1 | X2 | X3 | X4 | …… | ||||
| A | 80 | 74.5 | 15 | 16.0 | 5 | 5.8 | 5 | 6.3 | …… |
| D | 0 | 0 | 15 | 13.1 | 0 | 0 | 5 | 4.2 | …… |
| E | 0 | 0 | 5 | 4.7 | 5 | 4.6 | 0 | 0 | …… |
| F | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3.6 | …… |
| G | 3 | 3.5 | 15 | 14.1 | 5 | 5.2 | 15 | 15.4 | …… |
| H | 0 | 0 | 0 | 0 | 3 | 3.7 | 5 | 5.8 | …… |
| I | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3.5 | …… |
| K | 0 | 0 | 4 | 3.4 | 5 | 3.7 | 0 | 0 | …… |
| L | 0 | 0 | 6 | 6.3 | 10 | 9.1 | 8 | 7.2 | …… |
| M | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | …… |
| N | 0 | 0 | 0 | 0 | 5 | 4.7 | 5 | 4.6 | …… |
| P | 0 | 0 | 0 | 0 | 10 | 10.9 | 5 | 5.5 | …… |
| Q | 0 | 0 | 0 | 0 | 3 | 3.5 | 3 | 3.7 | …… |
| R | 3 | 3.1 | 15 | 17.7 | 20 | 21.7 | 10 | 12.0 | …… |
| S | 3 | 4 | 10 | 9.0 | 8 | 7.5 | 15 | 14.3 | …… |
| T | 8 | 10.6 | 0 | 0 | 10 | 8.3 | 5 | 4.6 | …… |
| V | 3 | 4.3 | 10 | 10.9 | 3 | 3.5 | 4 | 4.2 | …… |
| W | 0 | 0 | 0 | 0 | 3 | 3.2 | 0 | 0 | …… |
| Y | 0 | 0 | 5 | 4.7 | 5 | 4.5 | 5 | 5.4 | …… |
Table 2. The actual standard deviation of trimer oligos provided by Synbio Technologies
| The Requested Frequency(%) | The Actual Standard Deviation(%) |
|---|---|
| 1%-5% | 0.932 |
| 6%-10% | 0.577 |
| 10%-20% | 1.059 |
| 20%-40% | 3.551 |
| 40%-90% | 3.755 |
*The deviation between the actual proportion of trimer oligos delivered by Synbio Technologies and the proportion required by customers is small. This proves that the quality of trimer oligos delivered is high.
sgRNA Library Oligos
Synbio Technologies provides one-stop gene function screening services based on the CRISPR-Cas9 sgRNA library system. With our proprietary Syno 3.0 DNA synthesis platform, we can design and synthesize high quality sgRNA library oligos in a fast and efficient manner to meet the needs of customers for sgRNA library construction.
| Service Types | Turnaround Time (Business Days) | Price |
|---|---|---|
| CRISPR-Cas9 based oligo design | 1-3 | Inquire |
| CRISPR-Cas9 based oligo synthesis | Inquire |
Deliverables
Lyophilized dry DNA oligo powder
Certificate of Analysis (COA)
FAQs
What are the advantages of Oligo Pools over traditional oligo synthesis?
Traditional oligo synthesis is costly, time-consuming, and inefficient. Oligo Pools are high-throughput with high sequence coverage.
What do you need to watch out for during Trimer oligo synthesis experiments?
You need to keep an eye out for the GC content. The G+C content should be between 40% and 60%, with 45% to 55% being optimal.
You also need to be careful about the Tm value. The Tm value of the template position sequence corresponding to the primer should be around 72°C. This helps to optimize the PCR reaction conditions.
You should store trimer oligos at -20℃.
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