Synbio Technologies는 혁신적인 합성 생물학 솔루션을 제공하여 생명과학 연구 및 개발을 지원하는 기업입니다.
The General Introduction of
In Vitro Transcription
In vitro transcription takes linear DNA sequences as a template, using T7, T3, or SP6 RNA polymerase to synthesize RNA from the DNA template sequence.
Synbio Technologies offers efficient and cost-effective in vitro transcription RNA synthesis through our Syno synthesis platform. Our customers can provide us with the DNA templates, such as plasmid and/or PCR products, or we can conduct the de novo gene synthesis of the target DNA sequences. After codon optimization, 5‘-capping, 3’-addition and nucleoside modification, the transcribed RNA is suitable for gene editing, gene function research, gene diagnosis, structural research and nucleic acid vaccine development.
Highlights
One-Stop Service from DNA Template Synthesis to IVT RNA
Fully Customized Synthesis
Guaranteed Service: No RNA, No Pay
Free Codon Optimization
> 95% Purity & Strict QC
Abundant RNA Types
Service Details
| Size/Scale | PolyA Tails | Cap | Modifications | Purifications | Turnaround Time | Price |
|---|---|---|---|---|---|---|
| 40-9000 nt 2 µg-g | • Customized tail • 100-150A tail (enzymatic method) • 120A tail • No tail | • One stop capping (Clean cap AG, ARCA cap, m7GpppG Cap) • Enzymatic capping (Cap 0, 1) • No cap | • 5-mUTP • 5-moUTP • ψ-UTP • N1-Me-ψ-UTP • Cy3, Cy5, AF488 • Unmodified | • Silica column purification • LiCl purification • Phenol/chloroform extraction • HPLC | IVT: 5-10 BD Gene Synthesis: 10-15 BD | 2 µg start from $200 1 mg start from $1,000 |
Quality Control
| QC Items* | Method | Specifications | Default QC | QC Price |
|---|---|---|---|---|
| Appearance | Visual inspection | Clear, colorless, free from visible particulates | √ | Free |
| A 260/280 Ratio | UV Absorbance | 1.70 - 2.30 | √ | Free |
| Quantity | UV Absorbance | Quantity is ± 5% | √ | Free |
| RNA size & purity* | Denaturing agarose gel electrophoresis | Detect expected size band | √ | Free |
| Endotoxin | TAL assay | < 10 EU/mg | X | $50 per reaction |
| DNA template residue test | PCR & Gel electrophoresis | PCR product from the RNA products is <5% compared to positive control | X | $125 per reaction |
| Bioburden | Streak inoculation | No growth after 48 hours | X | $50 per reaction |
| Mycoplasma | Mycoplasma PCR Detection | No mycoplasma is detected in the RNA products | X | $125 per reaction |
| RNA Sequence confirm | RT-PCR & Sanger sequencing | Sequencing results are consistent with the confirmed RNA sequence | X | Starting at $250 |
| Functional test | Transfection & Western Blot | Detect expected size protein | X | Quote |
Workflow
Templates For In Vitro Transcription
dsDNA templates often increase transcription yield of the RNA. Synbio Technologies provides multiple types of DNA templates for in vitro transcription, including the linearized DNA plasmids, PCR products, synthetic oligos, and cDNA.
Deliverables
Liquid RNA
COA
Lyophilized template plasmid
Case Study
RNA (1010 nt) on denaturing agarose gel. The RNA was synthesized by in vitro transcription and purified via LiCl purification method.
FAQs
How do I ensure that the synthesized mRNA has high purity?
High purity mRNA can be obtained by optimizing in vitro transcription conditions and using efficient purification methods (e.g. column chromatography, gel electrophoresis, etc.).
Why is 5‘ capping and 3’ tailing required for mRNA synthesis?
5‘ capping and 3’ tailing help to improve the stability and translation efficiency of mRNA and prevent it from being degraded inside the cell.
How should I store my RNA products?
mRNA can remain stable for at least one year if stored at -80°C and not subjected to repeated freeze-thaw cycles, though stability varies with mRNA length. For long-term storage, keep mRNA at -80°C; for short-term, -20°C is recommended.
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Oligonucleotide Diagnostics & Therapeutics
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