Signalway Antibody (SAB)는 생명과학 연구 및 약물 개발을 지원하기 위해 고품질 항체, 단백질, 및 키트를 제공하는 글로벌 생명공학 기업입니다.

제품 설명

AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody

제품 번호

11501-1 / size: 50ul

11501-2 / size: 100ul

제품 특징

• Product NameAKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.
• Target NameAKT1/AKT2/AKT3
• ModificationPhospho
• Alternative Names    RAC-PK-alpha; Protein kinase B;
• Accession No. Swiss-Prot: P31749 P31751 Q9Y243
  NCBI Protein: NP_001014431.1 NP_001617.1 NP_005456.1
• UniprotP31749
• Gene ID207;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Background

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738.
Malabarba M G, et al. (1996) Biochem. J. 319:865-872.
Hou J, et al. (1994) Science. 265:1701-1706.
Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.

Published Papers

Enhancement of anti-acne effect of Scutellaria baicalensis extract by fermentation with symbiotic fungus Penicillium decumbens. In J Biosci Bioeng on 2020 Nov by Xiaojing Zhu, Yue Mao, et al..PMID: 32747300, , (2020),
PMID: 32747300Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway.In Mol Med Rep.On 2016 Oct by Gu H, Ji R et al..PMID:27513187, , (2016),
PMID: 27513187Please let us know if you have published research using #11501 so that we can cite your reference.

Images

• Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody #11501.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3(Phospho-Tyr315/316/312) Antibody #11501(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody #11501.
• Western blot analysis of extracts from 3T3 cells, treated with EGF or calf intestinal phosphatase (CIP), using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) Antibody #11501.
• Western blot analysis of extracts of various cell lines, using AKT1/AKT2/AKT3(phospho-Tyr315/316/312) Antibody

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