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제품 설명

Double-stranded RNA (dsRNA) ELISA kit

제품 번호

DEIA-BZ002P

제품 특징

Sample

vaccines and gene therapies

Species Reactivity

Universal

Detection Method

Sandwich-ELISA

Intended Use

This kit is used for quantitative detection of double stranded RNA (dsRNA) content in samples. The detected dsRNA is 40 bp or longer and is independent of its nucleic acid sequence.

Contents of Kit

Storage

1. For unused kit: The whole kit could be stored at 2-8°C in shelf life. Strong light should be avoided for storage stability.

2. For used kit: Once the microplate is opened, please cover unused wells with plate sealer and return to the foil pouch containing the desiccant pack, zip-seal the foil pouch and return to 2-8°C as soon as possible after use. Other reagents should be returned to 2-8°C as soon as possible after use.

Precision

CV of Intra-Assay ≤10%,CV of Inter-Assay ≤10%

Sensitivity

Limit of Detection
 Unmodified dsRNA Standard (5ng/μL): ≤0.001pg/μL
 pUTP-modified dsRNA Standard (5ng/μL): ≤0.001pg/μL
 N1-Me-pUTP-modified dsRNA Standard: ≤0.001pg/μL
 5-OMe-UTP-modified dsRNA Standard (5ng/μL): ≤0.01pg/μL
 

Limit of Quantitation
 Unmodified dsRNA Standard (5ng/μL): 0.0156pg/μL
 pUTP-modified dsRNA Standard (5ng/μL): 0.0156pg/μL
 N1-Me-pUTP-modified dsRNA Standard: 0.0312pg/μL
 5-OMe-UTP-modified dsRNA Standard (5ng/μL): 0.0625pg/μL

General Description

mRNA has gained significant worldwide attention as a novel active ingredient in vaccines and gene therapies. The increasing demand for mRNA molecules has compelled mRNA manufacturers to quickly scale up production capacity while maintaining high mRNA quality. In vitro transcription by T7 polymerase is the standard procedure to synthesize mRNA. However, this procedure may introduce double-stranded RNA (dsRNA) contaminants from random priming of abortive transcripts, turn-around transcription, and antisense transcription. dsRNA immune activation results in the up-regulation of various pro-inflammatory cytokines, and cell death, which can lead to patient morbidity. Therefore, to improve the quality of mRNA translation, and minimize adverse effects, it is critical to carefully monitor in vitro-transcribed (IVT) mRNA products and confirm the removal of dsRNA after purification.

Standard Curve

Citations

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