Gentarget는 유전자 편집 및 생명과학 연구를 위한 고급 솔루션과 서비스를 제공하는 글로벌 생명과학 기업입니다.
SEAP (Secreted Embryonic Alkaline Phosphatase) is a truncated form of human placental alkaline phosphatase (PLAP) through the deletion of a GPI anchor. It is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters, like luciferase. It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.
The SEAP is commonly used as a powerful reporter gene for the investigation of promoter activity in transfected eukaryotic cells. The SEAP expression level (reflect its promoter activity) can be detected via chemiluminescent reporter assay based on dioxetane CSPD (chloro-5-substituted adamantyl-1,2-dioxetane phosphate), and provides a convenient and highly sensitive method for the quantitation of transcriptional activity.
GenTarget provides Pre-made lentivirus expressing SEAP under different promoters (TetCMV, EF1a, CAG, Ubc, mPGK, Actin-beta or a signal pathway responsive promoter), containing an antibiotic selection (Puromycin, Blasticidin, Neomycin, Hygromycin, Zeocin), or a fluorescent marker (GFP, RFP), or fluorescent-antibiotic fusion dual selection. The lentivector’s core structure scheme is presented in the picture below:
See each product below. Click Product Manual for product list.
SEAP Reporter
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