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Abinscience

[Abinscience] Human IL21 Recombinant Protein (C-His)

Cat-No. HV658012



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Human IL21 Recombinant Protein (C-His)




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HV658012




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Catalog No.HV658012
Description




Recombinant Human IL21 Protein, C-His (HV658012) expressed in E. coli, spanning Leu23-Ser162. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9HBE4
Protein lengthLeu23-Ser162
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
NatureRecombinant
Endotoxin levelPlease contact with the lab for this information.
Purity>90% as determined by SDS-PAGE.
Predicted molecular weight17.45 kDa
FormLyophilized
Storage bufferLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

ReconstitutionReconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
ShippingIn general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesIL-21, Interleukin-21, IL21, Za11
Background

Interleukin-21 (IL21) is a ~18 kDa protein. Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection.

1. Parrish-Novak, J. et al. (2000) Nature 408, 57-63. PMID: 11081504
2. Strengell, M. et al. (2004) Journal of leukocyte biology 76, 416-22. PMID: 15178704
NoteFor research use only.







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