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제품 설명
Human CCL11/Eotaxin Recombinant Protein (N-His-SUMO)
제품 번호
HW762022
제품 특징
| Catalog No. | HW762022 |
|---|---|
| Description | Recombinant Human CCL11/Eotaxin Protein, N-His-SUMO (HW762022) expressed in E. coli, spanning Ile41-Pro97. Purity: >90% by SDS-PAGE. Highlights
|
| Expression system | E. coli |
| Accession | P51671 |
| Protein length | Ile41-Pro97 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 18.93 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | Small-inducible cytokine A11, SCYA11, Eotaxin, CCL11, Eosinophil chemotactic protein, C-C motif chemokine 11 |
| Background | Eotaxin (CCL11) is a ~10 kDa protein. Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells. Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway. 1. Garcia-Zepeda, EA. et al. (1996) Nature medicine 2, 449-56. PMID: 8597956 2. Wakabayashi, K. et al. (2021) Scientific reports 11, 7937. PMID: 33846499 3. Kitaura, M. et al. (1996) The Journal of biological chemistry 271, 7725-30. PMID: 8631813 4. Park, JY. et al. (2017) Journal of receptor and signal transduction research 37, 416-421. PMID: 28279120 |
| Note | For research use only. |
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