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제품 설명 

Anti-Hypusine antibody (Hpu24)

제품 번호

RGK08101

제품 특징

Catalog No.

RGK08101

Expression system

Mammalian Cells

Species reactivity

General

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

ELISA: 1:2000-1:8000, IHC: 1:50-1:100, WB: 1:500-1:4000

Target

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

PubChem: 65396

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Hpu24

Data Image

• 

  Western Blot
  Various lysates were subjected to SDS PAGE followed by western blot with Hypusine antibody (RGK08101) at 1ug/ml.
  
  Lane 1: Hela cell lysate
  Lane 2: HepG2 cell lysate
  Lane 3: K562 cell lysate
  Lane 4: PC-12 cell lysate
  Lane 5: Jurkat cell lysate
  Lane 6: MCF-7 cell lysate
  Lane 7: 293T cell lysate
  
  Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
  
  Predict MW: 17 kDa
  Observed MW: 17 KDa
• 

  SEC-HPLC
  The purity of this product is >95% as determined by SEC-HPLC.

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