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YEASEN

[YEASEN] mRNA Cap 2'-O-Methyltransferase GMP-grade (50 U/μl)

Cat-No. 10612ES

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.

제품 설명

mRNA Cap 2'-O-Methyltransferase GMP-grade (50 U/μl)

제품 번호

10612ES

제품 설명

Description

mRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.
This product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo/in vitro.

Features

Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, Rnases

Applications

2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo
mRNA cap-1 structure may improve expression

Specifications

Source	Recombinant E.coli with Cap 2´-O-Methyltransferase gene
Optimum Temperature	37℃
Storage Buffer	20 mM Tris-HCl pH8.0, 0.1mM EDTA, 1mM DTT, 100mM NaCl, 50% (v/v) glycerin, 0.1% (v/v) Trion X-100
Unit Definition	One unit is defined as the amount of enzyme required to methylate 10 pmol of 80nt capped RNA transcript in 1 h at 37°C.

Components

Components No.	Name	10612ES92 (10,000 U)	10612ES97 (50,000 U)	10612ES98 (250,000 U)	10612ES99 (20 MU)
10612	mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U/μl)	200 μL	1 mL	5 mL	400 mL