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[BosterBio] Anti-Ltb Antibody Picoband®

Cat-No. A02565-2



Boster Bio는 생명과학 연구를 위한 항체, ELISA 키트, 단백질 및 관련 제품을 제공하는 미국의 생명과학 기업입니다. 




제품 설명


Anti-Ltb Antibody Picoband®



제품 번호


A02565-2



제품 특징


Ltb antibody (A02565-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 1 Publication


Flow Cytometry analysis of RAW264.7 cells using anti-Ltb antibody (A02565-2).
Overlay histogram showing RAW264.7 cells stained with A02565-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ltb Antibody (A02565-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.


Western blot analysis of Ltb using anti-Ltb antibody (A02565-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse Raw264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ltb antigen affinity purified


Reactive SpeciesHostApplication
Mouse, RatRabbitELISA, Flow Cytometry, WB


Product Information

Product Name
Anti-Ltb Antibody Picoband®
View all LTB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived mouse Ltb recombinant protein (Position: R68-G306).
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
25 kDa
Calculated Molecular Weight

32.3 kDa

Background of Ltb
Lymphotoxin-beta (LT-beta) also known as tumor necrosis factor C (TNF-C) is a protein that in humans is encoded by the LTB gene. Lymphotoxin beta is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-Ltb Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02565-2)




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