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Cusabio

[Cusabio] Human Fibrinogen(FB) ELISA Kit

Cat-No. CSB-E13656h


CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체, 

10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다. 




제품 설명


Human Fibrinogen(FB) ELISA Kit




제품 번호


CSB-E13656h




제품 특징


Product Details

Target Name
Fibrinogen(FB)
Alternative Names
N/A
Abbreviation
Fb
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 μg/mL-20 μg/mL
Sensitivity
0.078 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %85
Range %80-92
1:200Average %98
Range %90-105
1:400Average %97
Range %90-105
1:800Average %93
Range %86-98
Recovery
The recovery of human FB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Serum (n=5)9287-98
EDTA plasma (n=4)9890-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
202.6482.7012.6752.583
102.1592.1692.1642.072
51.4761.5361.5061.414
2.50.8640.8760.8700.778
1.250.4430.4750.4590.367
0.6250.2340.2530.2440.152
0.3120.1460.1540.1500.058
00.0950.0890.092 
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Fibrinogen (FB) ELISA Kit was designed for the quantitative measurement of Human Fibrinogen (FB) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 μg/mL-20 μg/mL and the sensitivity is 0.078 μg/mL.



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