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Cusabio

[Cusabio] Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

Cat-No. CSB-E17116h


CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체, 

10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다. 




제품 설명


Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit




제품 번호


CSB-E17116h




제품 특징


Product Details

Target Name
Collagen Type Ⅳ(COL Ⅳ)
Alternative Names
Collagen Type 4(COL 4),Collagen Type IV(COL IV)
Abbreviation
Col Ⅳ
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %86
Range %80-104
1:400Average %97
Range %91-105
1:800Average %100
Range %92-113
1:1600Average %93
Range %86-98
Recovery
The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Serum (n=5)9689-102
EDTA plasma (n=4)9385-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.7482.8562.8022.635
20002.4592.4362.4482.281
10001.8761.9681.9221.755
5001.2401.2341.2371.070
2500.8430.8360.8400.673
1250.4340.4470.4410.274
62.50.2460.2560.2510.084
00.1650.1680.167 
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Col Ⅳ ELISA Kit was designed for the quantitative measurement of Human Col Ⅳ protein in serum, plasma, cell culture supernates, urine. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .



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