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Cusabio

[Cusabio] Pig Ghrelin (GHRL) ELISA kit

Cat-No. CSB-E09437p


CUSABIO는 암, 세포 생물학, 면역학, 신경과학, 후생유전학 등 연구 분야의 글로벌 고객에게 60,000개 이상의 검증된 항체, 

10,000개 이상의 재조합 단백질, 660개 이상의 사이토카인 및 수천 개의 ELISA 키트를 제공하는 검증된 제조업체 입니다. 




제품 설명


Pig Ghrelin (GHRL) ELISA kit




제품 번호


CSB-E09437p




제품 특징


Product Details

Target Name
Ghrelin
Alternative Names
GHRLAppetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin] ELISA Kit
Abbreviation
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

SampleSerum(n=4)
1:1Average %93
Range %89-97
1:2Average %99
Range %94-103
1:4Average %106
Range %100-114
1:8Average %95
Range %90-100
Recovery
The recovery of pig GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)8480-88
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.4812.5832.5322.382
20001.8731.9041.8891.739
10001.2931.2211.2571.107
5000.7340.7080.7210.571
2500.4270.4620.4450.295
1250.3220.3020.3120.162
62.50.2500.2590.2550.105
00.1540.1460.150
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig GHRL ELISA Kit was designed for the quantitative measurement of Pig GHRL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.



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