(주)모아바이오

Minerva Biolabs

[Minerva Biolabs] Venor®GeM Classic

Cat-No. 11-1025

11-1025 / size: 25 reactions
11-1050 / size: 50 reactions
11-1100 / size: 100 reactions
11-1250 / size: 250 reactions

Minerva Biolabs는 세포배양 및 바이오의약품에서의 mycoplasma, bacteria, virus를 검출/오염방지/제거 등을

컨트롤할 수 있는 고품질의 다양한 키트와 제품을 제공하는 독일 소재의 제조사입니다.

제품 설명

Venor®GeM Classic

제품 번호

11-1025 / size: 25 reactions

11-1050 / size: 50 reactions

11-1100 / size: 100 reactions

11-1250 / size: 250 reactions

제품 특징

Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR. All Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) species so far described as contaminants of cell cultures and media components are specifically detected by amplification of a highly conserved rRNA operon, or more specifically, of the 16S rRNA coding region in the mycoplasma genome. The mycoplasma-specific amplification is detected at 265-278 bp. False negative results due to PCR inhibitors or improper DNA extraction can be ruled out by using the provided internal amplification control, which is detected at 191 bp.

Type of PCR

Conventional, endpoint PCR

Recommended Use

Applicable in research and industry for the direct detection of Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) contaminations in cell cultures and biopharmaceuticals. Approved for testing according to EP 2.6.7 and JP G3. Intended for research use only. Not recommended for clinical diagnostics.

Kit Components

Lyophilized Mix: Primer sets / Nucleotides in aliquots of 25 reactions each
10x Reaction Buffer (optimized for MB Taq DNA Polymerase)
Lyophilized Positive Control DNA
Lyophilized Internal Control DNA
PCR Grade Water

Package Sizes

Cat. No. 11-102525 Reactions
Cat. No. 11-105050 Reactions
Cat. No. 11-1100100 Reactions
Cat. No. 11-1250250 Reactions

Result Evaluation

Gel electrophoresis at endpoint of PCR

Required Consumables

Taq DNA Polymerase. We highly recommend our reliable hot-start MB Taq DNA Polymerase, Cat. No. 53-0050/-0100/-0200/-0250 or 53-1050/-1100/-1200/-1250.
PCR reaction tubes
Requirement for EP 2.6.7 / JP G3 compliant testing:
DNA extraction kit, e.g. Venor®GeM Sample Preparation Kit (Cat. No. 56-1010/50/200)
Optional for process validation and EP 2.6.7 / JP G3 compliant testing:
Internal Control DNA extra (4 vials for 300 µl each of internal amplification control; Cat. No. 11-1905).
10CFU™ Sensitivity Standards and 100CFU™ Sensitivity Standards available for all EP/JP listed mycoplasma species.

Required Lab Devices

PCR cycler for conventional PCR
Agarose gel electrophoresis and DNA staining system
Pipetting equipment
Tube centrifuge

Shelf Life and Storage

Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.

EP 2.6.7 / JP G3 compliance

Use of Venor®GeM Classic for QA testing of biologicals like master and working cell banks, autologous cells, culture media, bulk harvest and final product testing according to EP 2.6.7 and JP G3 is applicable after appropriate sample preparation and process validation.