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YEASEN

[YEASEN] DH5α Fast Chemically Competent Cell

Cat-No. 11803ES80

YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.




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DH5α Fast Chemically Competent Cell




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11803ES80 / size: 10×100 μL




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Description

DH5α Fast Chemically Competent Cell from YEASEN is a competeny cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with the conversion efficiency up to 10cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification, and can ensure stable inheritance of high-copy plasmids. At the same time, it can be used to construt clones, blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA. F-DH5α competent cells were prepared by special technology, without heat shock at 42℃ or incubated at 37℃, only 10 minutes to complete a transformation.

DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk-,mk+) supE44λ- thi -1 gyrA96 relA1phoA

Features

  1. High transformation efficiency: 1×108 cfu/μg pUC19 DNA
  2. Genetic markers that allow for blue/white colony screening
  3. Rapid: It only takes 10 minutes to complete one transformation

Applications

  1. Efficient DNA cloning derived from PCR, cDNA-generation reactions
  2. recA1 marker facilitates working with difficult to transform DNA


Specifications

Species

Escherichia coil

Cell type

Chemical competent cell

Whether it contains an F’ episome

No

Efficiency

1×108

Blue/white colony screening

Yes

Bacterial or yeast strains

DH5α

Amplification of the ccdB-containing vector

It is not suitable for amplification of ccdB-containing vectors


Components

Components No.

Name

11803ES80

11803-A

DH5α Fast Chemically Competent Cell

10×100 μL

11803-B

pUC19 (control vector,10 pg/μL)

10 μL


Shipping and Storage

The product is shipped with dry ice and can be stored at -85℃~-65℃ for six months. Do not store the product in -20℃ or liquid nitrogen.

 

                                                                                                                                                                                                                                                                                                                                                                                   Ver.EN20230928



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