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Biotechrabbit

[Biotechrabbit] PCR Grade Water

Cat-No. BR1900301


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


PCR Grade Water



제품 번호


BR1900301 - 15ml

BR1900302 - 300ml


제품 특징


Features

  • Ultrapure sterile filtrated deionized water
  • Prepared without the use of chemicals such as DEPC
  • DNase-, RNase- and Protease-free
  • Bioburden tested

Applications

  • Ready-to-use water for standard and enhanced PCR and qPCR
  • Buffer preparation and enzyme dilutions
  • All other molecular biology applications


Description


biotechrabbit™ PCR Grade Water is ultrapure and sterile filtered deionized water prepared by a proprietary process without the use of chemicals such as DEPC (diethylpyrocarbonate). The water is free from DNase, RNase, protease and bioburdon contaminations, for use in PCR and all other molecular biology applications.


Other Information


Component

Composition

PCR Grade Water

Ultrapure sterile filtered deionized water.

 

 

 

STORAGE

Room temperature (unopened)

-20°C (until expiry date – see product label)



Quality Control
RNase activity

Absence of RNase activity was checked by incubation of RNA in PCR Grade Water at 37°C for 3 hours. No degradation detected.

DNase activity

PCR Grade Water was incubated with DNA fragments and Plasmid DNA for 14 – 16 hours at 37°C. Samples were checked on an agarose gel. No degradation detected.

Protease activity

A reference protein was incubated in PCR Grade Water for 3 days at 37°C. Samples were checked on a polyacrylamide gel. No degradation detected.

Bioburden

The number of viable aerobic microorganisms present in PCR Grade Water was tested using membrane filtration and incubation for 3-5 days. No growth detected.


Protocols

PCR Recommended use
Prevention of PCR or reverse transcription contamination

When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA or RNA.

  • Use separate clean areas for preparation of samples and reaction mixtures and for cycling.
  • Wear fresh gloves. Use sterile tubes and pipette tips with aerosol filters for PCR or reverse transcription setup.
  • Use only water and reagents that are free of DNA and nucleases.
  • With every assay setup, perform a contamination control reaction that does not include template DNA or RNA.



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Biotechrabbit - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"

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