biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.

제품 설명

4X CAPITAL™ 1-Step qRT-PCR Probe Master Mix

제품 번호

BC0503202 

제품 특징

LYO Master Mix RECONSTITUTION

1) Transfer 400 µl of the 5X qRT-PCR Probe Reconstitution Buffer to one vial Lyo CAPITAL qRT-PCR Probe Mix. Discard the remaining overfill.
2) Mix well – the lyophilisate will dissolve within seconds
3) Store the reconstituted CAPITAL qRT-PCR Probe Mix at -20°C

STORAGE

Store at room temperature or below (until expiry date – see product label)
Reconstituted lyophilisate: store at −20°C for up to 12 months

Quality Control Functional assay

Mix tested functionally in qRT-PCR.

Basic Protocol

• Keep the master mix protected from light until you use it.
  
  
• Aliquot the master mix to minimize freeze-thaw cycles and light exposure.
  
  
• Thaw on ice and mix very well all reagents. Assemble and keep all reactions on ice.
  
  
• Use only high quality optically clear reaction plates and seals designed for fluorescence applications.
  
  
• Do not use corner wells or use a more robust seal.
  
  
• Reserve plate positions for positive (control DNA) and negative (water or buffer) controls.
  
  
• First pipette the primer mixture, then add the template and last the Master Mix.
  
  
• Before preparing mixes, calculate the volume needed according to the reaction number plus one extra.
  
  
• To have a better correlation, run the reactions in triplets.

Biotechrabbit에서 다양한 제품을 찾아보세요! 

• PCR products
• 
  
• Lyophilized PCR Kits
• 
  
• Real Time PCR
• 
  
• Nucleotides
• 
  
• Nucleic Acid Purification
• 
  
• Electrophoresis Ladders
• 
  
• Enzymes and Proteins
• 
  
• Protein Research
• 
  
• Cell-free Protein Synthesis

Biotechrabbit - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"