Virongy는 바이러스 및 바이러스 벡터 연구를 위한 혁신적인 기술과 제품을 제공하는 미국의 제조업체 입니다.

Utilizing Our NEW Hybrid Alphavirus Particle Technology. 

Now with ready-to-use cells! No need to culture cells or CO2 incubators. 

Cells come pre-counted so just thaw and go.

제품정보

품명: Rapid Cell-Based SARS-CoV-2 Neutralizing Antibody Assay Kit

사이즈: 96 well Rapid Neutralizing Assay Kit - with Ready-To-Use Cells

제품구성:

- 5 mL of Ha-CoV-2 Pseudovirus Particles
- 1 Tube of Ready-To-Use Target Cells (5 Million Cells/Tube)
- 50 µL of Standard SARS-CoV-2 Neutralizing Antibody
- 15 mL of VBI Infection Medium
- 2 mL of Lysis Buffer
- 3.5 mL of Firefly Luciferase Assay Buffer

제품설명

The entry of SARS-CoV-2 into host cells is mediated by the trimeric transmembrane spike (S) glycoprotein. The S glycoprotein is surface-exposed and mediates entry into the host cells by binding to the human angiotensin-converting enzyme 2 receptor which makes the S glycoprotein a major target of neutralizing antibodies. Neutralizing antibodies blocking the S protein and receptor binding could prevent the virus from infecting host cells. Through the detection of the luminescence levels of target cells, it is possible to screen or verify the activity of these neutralizing antibodies.

Our product is fundamentally superior to any of our competitors due to the implementation of our newly developed Ha-CoV-2 particle. Ha-CoV-2 is a newly developed hybrid SARS-CoV-2 virus-like particle (VLP) that encapsulates an alphavirus-derived RNA genome for rapid expression of reporter genes (luciferase or GFP) in target cells (Hetrick et al., 2022). Different from commonly used S protein pseudotyped lenti- or vesicular stomatitis virus (VSV)-pseudoviruses, Ha-CoV-2 is assembled with all 4 structural proteins (S, M, N, and E) of SARS-CoV-2, and contains a reporter genome derived from an alphavirus-based vector for rapid (4 hours) and robust expression of reporter genes. Ha-CoV-2 represents a major technology advancement in the development of SARS-CoV-2 pseudoviruses, and serves as platforms for rapid and robust quantification of neutralizing antibodies, viral mutants, and antiviral drugs (Dabbagh et al., 2021; He et al., 2021).

Virongy offers pre-assembled Ha-CoV-2 reporter pseudovirions and derived S protein variants. We also help customers to perform antiviral drug screening and quantification of neutralizing antibodies.

• Ha-CoV-2 제품의 주요 특징
• 
  
  • ELISA 기반 방법보다 쉽고 간단한 프로토콜
  • 기존 ELISA 기반의 neutralization assay보다 더 높은 정확도
  • 다른 pseudovirus들에 비해 빠른 속도 – (최대 리포터 발현 시간은 12~18시간)
  • 더욱 강력한 reporter signal – Ha-CoV-2는 리포터 발현을 위해 alphavirus vector의 빠르고 강력한 유전자 발현 능력을 활용합니다.
  • 입자 구조의 높은 명확도 – Ha-CoV-2에는 SARS-CoV-2 구조 단백질 4개(S, M, E, N)가 모두 포함되어 있지만 다른 바이러스의 구조 단백질은 포함되어 있지 않습니다. 대조적으로, lneti- 및 VSV- peurovirus는 SARS-CoV-2의 S 단백질과 HIV-1 Gag 및 Pol과 같은 다른 바이러스의 여러 구조 단백질만을 포함합니다.
  • Strong correlation (coefficient value r2 = 0.87) with wild-type SARS-CoV-2 in neutralizing antibody assays (Hetrick et al., 2020).
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Example of results

* Customize your kit by selecting from our list of SARS-CoV-2 spike mutants or variants:

>>> 기타 SARS-CoV-2 spike mutants or variants 더보기 링크>>>