YEASEN은 생명과학 연구 및 진단을 위한 고품질의 연구용 시약과 기기를 제공하는 글로벌 바이오 기업입니다.
제품 설명
SuperSignal MaxiSignal Maximum Sensitivity Substrate
제품 번호
36224ES10 / size: 10ml
36224ES60 / size: 100ml
36224ES70 / size: 200ml
36224ES76 / size: 500ml
제품 설명
SuperSignal MaxiSignal Maximum Sensitivity Substrate is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.
This kit has a unique luminescent/enhanced substrate system, While reducing the exposure background, the introduction of a new oxidant greatly improves the stability of the kit, which can achieve low fick-grade western blotting detection. In addition to X-rays, can also be detected by the automatic imaging system.
Features
Applications
| Recommended Antibody Concentrations | primary antibody (liquid storage concentration 1mg/mL): 1:1000-1:100000 secondary antibody (liquid storage concentration 1mg/mL): 1:100000-1:500000 |
| Signal Duration | < 8 h |
| Substrate Properties | Chemical Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) Substrate |
| Sensitivity | low fick-grade antigens |
| Components No. | Name | 36224ES10(10 mL) | 36224ES60(100 mL) | 36224ES70(200 mL) | 36224ES76(500 mL) |
| 36224-A | MaxiSignal ECL - Reagent A | 5 mL | 50 mL | 100 mL | 250 mL |
| 36224-B | MaxiSignal ECL - Reagent B | 5 mL | 50 mL | 100 mL | 250 mL |
The product should be stored at 2℃~8℃ for one year.
Notes: 36224-A should be stored away from the light!
Figure 1. Comparison of detection effect between YEASEN Maxisignal substrate and West Femto of the same level substrate of other brands
YEASEN ECL reagent has a better use effect than other brands of the same level of products.
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