Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.
제품 설명
GenUP™ Micro RNA Kit
제품 번호
BR0701901 - 10 preps
BR0701902 - 50 preps
BR0701903 - 250 preps
제품 특징
Features
- Fast and simple procedure
- Optimized binding conditions for high yields of small RNAs
- Physical removal of DNA, no DNase treatment, no toxic β-mercaptoethanol
Applications
- Efficient isolation of snRNA, miRNA, siRNA, tRNA, rRNA and mRNA from various starting material
Description
biotechrabbit™ GenUP Micro RNA Kit is designed for high yields of small RNA molecules and total RNA, including snRNA, miRNA, siRNA, tRNA, rRNA and mRNA, without the use of highly toxic β-mercaptoethanol. After using well established filter-technology to selectively remove genomic DNA, the RNA is bound, washed and eluted from the filter membrane using RNase-free water.
Other Information
STARTING MATERIAL | Eukaryotic cells (5 × 106)
Fresh or frozen tissue or biopsies (up to 20 mg)
Gram-positive and Gram-negative bacteria (up to 1 × 109) | EXTRACTION TIME | Typically 15–40 minutes | BINDING CAPACITY | 100 µg | Yield | Depends on the type and the amount of the starting material | RECOVERY RATE | High rate of recovery for small RNA molecules |
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Protocols
STEPS |
- Homogenize and lyse the sample material.
- Centrifuge to pellet unlysed material.
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- Transfer the lysate to the Mini Filter DNA (blue).
- Centrifuge, and discard the Mini Filter DNA with bound DNA.
- The filtrate contains the RNA, don’t discard!
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- Add isopropanol to the filtrate.
- Transfer to a Mini Filter RNA (violet) to bind RNA and centrifuge.
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- Add Buffer WASH A and centrifuge.
- Add Buffer WASH B and centrifuge.
- Centrifuge once more to remove residual ethanol.
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- Add Water, RNase-free (for ELUTION), incubate and centrifuge.
- microRNA in the Elution Tube is ready for use.
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For detailed protocol, please, refer to the product manual (Related Documents).
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Biotechrabbit - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"
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