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Biotechrabbit

[Biotechrabbit] GenUP™ Blood RNA Kit

Cat-No. BR0701401


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ Blood RNA Kit

 


제품 번호


BR0701401 - 10 preps

BR0701402 - 50 preps

BR0701403 - 250 preps


제품 특징


Features

  • Fast and simple procedure
  • High quality RNA isolated from fresh and frozen, EDTA- or citrate-treated blood
  • Physical removal of DNA, no DNase treatment, no toxic β-mercaptoethanol

Applications

  • Isolation of up to 8 µg total RNA from 0.5–1.0 ml whole blood



Description


biotechrabbit™ GenUP Blood RNA Kit is designed for fast isolation of total RNA from up to 1 ml whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. Erythrocytes are removed in an initial lysis step. After an second lysis step, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. Including lysis, isolated RNA is available in approximately 45 min. The isolated RNA is suitable for a wide range of different molecular biology applications, including RT-PCR.

The GenUP Blood RNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP Total RNA Kit (BR07009), GenUP Virus RNA Kit (BR07010), GenUP Virus DNA/RNA Kit (BR07011) or UPzol RNA Isolation Solution (BR07001).



Other Information


STARTING MATERIAL

Fresh whole blood; stabilized with EDTA or citrate (0.5–1.0 ml)

EXTRACTION TIME

Approximately 45 min

BINDING CAPACITY

> 20 µg RNA

TYPICAL YIELD

Variable depending on the starting material; 1–8 µg RNA



Protocols

STEPS

  • Homogenize and lyse 0.5–1.0ml whole blood sample by adding Buffer LYSIS LE, incubate and centrifuge.
  • Transfer the lysate to the Mini Filter DNA (blue).
  • Centrifuge, and discard the Mini Filter DNA.
  • Add equal volume 70% ethanol and transfer the filtrate to the Mini Filter RNA (violet).
  • Centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Elute RNA with Water, RNase-free (for ELUTION) and centrifuge.
  • Purified RNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).









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