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Biotechrabbit

[Biotechrabbit] GenUP™ Virus DNA/RNA Kit

Cat-No. BR0701101


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ Virus DNA/RNA Kit



제품 번호


BR0701101 - 10 preps

BR0701102 - 50 preps


제품 특징


Features

  • Universal kit for simultaneous RNA and DNA isolation from different starting materials
  • Fast and simple procedure with sample-specific protocols
  • High yields of pure RNA and DNA
  • No DNase treatment, no toxic β-mercaptoethanol

Applications

  • Simultaneous viral DNA and RNA isolation from various sources
  • Excellent performance for unknown viruses



Description


biotechrabbit™ GenUP Virus DNA/RNA Kit has been specially developed for quick and easy isolation of viral RNA and DNA. The kit is especially useful when the origin of the virus is unknown. Viral double-stranded DNA and single-stranded RNA are simultaneously isolated from eukaryotic samples, including plasma, serum, and other body fluids as well as cell cultures, tissues, and buccal swabs.

The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified nucleic acid can be achieved with flexible elution volumes. The kit includes carrier RNA.

After a few initial procedures, the viral nucleic acids are bound to a Mini Filter, washed and then eluted in a separate tube. The purified nucleic acids are ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis, qPCR and RT-PCR.



Other Information


STARTING MATERIAL

Eukaryotic cells (up to 5 × 106)
Serum, plasma, cell-free body fluids, cell culture supernatants (150 µl)
Tissue samples, biopsies (up to 20 mg)
Paraffin-embedded tissues, buccal swabs

EXTRACTION TIME

Approximately 25 min

TYPICAL YIELD

Yield is highly dependent on sample type



Protocols

STEPS

  • Homogenize and lyse the sample material.
  • Centrifuge to pellet debris.
  • Add Buffer BINDING BD and transfer to a Mini Filter (blue).
  • Centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add Water, RNase-free (for ELUTION), incubate and centrifuge.
  • DNA/RNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).




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Biotechrabbit - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"

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