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Antibody System

[Antibodysystem] Anti-Human GAPDH Polyclonal Antibody

Cat-No. PHC09001



AntibodySystem는 Primary Antibody Biosimilar - Research Grade 

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제품 설명 


Anti-Human GAPDH Polyclonal Antibody

 



제품 번호


PHC09001




제품 특징

Catalog No.

PHC09001

Species reactivity

Human, Mouse, Rat, Zebrafish

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GAPDH (Met1-Glu335).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase

Purification

Purified by antigen affinity column.

Accession

P04406

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.




Data Image


  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with GAPDH antibody (PHC09001) at 1μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: PC-3 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: MCF-7 cell lysate
    Lane 8: HT-29 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 36 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse intestine stained for GAPDH with PHC09001.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GAPDH antibody (PHC09001) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 38 kDa
    Observed MW: 38 kDa



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