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제품 설명
Anti-Human GAPDH Polyclonal Antibody
제품 번호
PHC09001
제품 특징
Catalog No.
PHC09001
Species reactivity
Human, Mouse, Rat, Zebrafish
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human GAPDH (Met1-Glu335).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase
Purification
Purified by antigen affinity column.
Accession
P04406
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
Various lysates were subjected to SDS PAGE followed by western blot with GAPDH antibody (PHC09001) at 1μg/ml.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: K562 cell lysate
Lane 5: PC-3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 36 kDa
Observed MW: 36 kDa
Immunohistochemical analysis of mouse intestine stained for GAPDH with PHC09001.
Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GAPDH antibody (PHC09001) at 1 µg/ml.
Lane 1: Recombinant Protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 38 kDa
Observed MW: 38 kDa
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