Cohesion Biosciences는 Primary Antibodies, SiRNA, Assay Kits, ELISA Kits, Protein, Enzyme등
수많은 High-Quailty 제품군을 전문 생산하는 미국의 Biological 제조사입니다
제품 설명
제품 번호
Cat. CRM4689
Size: 15nmol, 30nmol
제품 특징
Description: siRNA to inhibit CD97 expression using RNA interference
Form: Lyophilized powder
Gene Symbol: ADGRE5
Alternative Names: CD97 antigen; CD antigen CD97
Entrez Gene (Mouse) : 26364
SwissProt (Mouse) : Q9Z0M6
Purity : > 97%
Directions for Use : We recommends transfection with 10 nM - 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For example, resuspend one tube of 5 nmol siRNA oligo in 250 μl of DEPC water to get a final concentration of 20 μM.
Quality Control : Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Components : We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CD97 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot.
Storage/Stability : Shipped at 4 °C. Store at -20 °C for one year.
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