Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록
폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.
제품 설명
KRAS G12D Mutant Recombinant Antibody [29B]
제품 번호
ATM00004
제품 특징
Product Info
Product Info| Clonality | Recombinant Monoclonal |
|---|
| Clone | 29B |
|---|
| Isotype | IgG |
|---|
| Host | Rabbit |
|---|
| Application | WB,IF |
|---|
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
|---|
| Immunogen | Recombinant Human KRAS G12D Mutant aa2-128 |
|---|
| Purification | Protein A |
|---|
| Concentration | 1 mg/mL |
|---|
Target Info
| Gene Symbol | KRAS G12D Mutant |
|---|
| Gene Full Name | KRAS |
|---|
| Alias Symbols | NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2 |
|---|
| NCBI Gene Id | 3845 |
|---|
| Protein Name | GTPase KRas |
|---|
| Description of Target | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. |
|---|
| Uniprot ID | P01116-1 |
|---|
| Molecular Weight | 21.7 kDa |
|---|
40 µg of whole cell lysate or 500 ng of in vitro expressed G12 WT and mutants were probed with KRAS G12D Mutant Recombinant Antibody [29B] (ATM00004) at 3 µg/ml.
KRAS G12D Mutant Recombinant Antibody [29B] (ATM00004) staining of PANC-1 (KRAS G12 Heterozygous), THP-1 (NRAS G12D Heterozygous) and HEK293 KRAS WT. Methanol fixed cells were probed with recAb at 1/50 dilution (20ug/ml) and visualized using anti Rabbit AF488
Surface-attached KRAS G12D Mutant Recombinant Antibody [29B] (ATM00004) at 50 µg/ml facilitated kinetic analysis via LSA Kinetic software, with two G12D antigen concentrations showing association and dissociation rates, ka = 2.7×10^3 M^-1s^-1 and kd = 1.4×10^-4 s^-1, respectively, and a 50 nM affinity constant (KD). No specific binding was observed for KRAS antigens with G12G or mutations G12S, G12C, G12R, and G12A
KRAS G12D Mutant Recombinant Antibody [29B] (ATM00004) rabbit Ab binding kinetics vs Human Leukocyte Antigen HLA-A11:01&B2M presenting KRAS- G12D peptide in 1x PBS buffer, pH 7.0. Serial dilutions of KRAS G12D Mutant Recombinant Antibody [29B] (ATM00004) rabbit antibodies from 50 ug/mL to 1.6ug/mL were used to attach the KRAS G12D Mutant Recombinant Antibody [29B] (ATM00004) to the surface for kinetic analysis via LSA Kinetic software fitting algorithm, with two G12D antigen concentrations showing association and dissociation rates, ka =2.78x10^3 M-1s-1 and kd = 5.83x10-5 s-1, respectively, and a 23.4 nM affinity constant (KD). Sequential testing with 50 nM and 100 nM of G12D antigens yielded these kinetic parameters, represented by blue and green curves. No specific binding was observed for KRAS antigens with G12G or mutations G12S, G12C, G12R, and G12A
Aviva Systems Biology의 모든 제품을 만나 보세요!
Products
New Product Releases
ELISA Kits
Polyclonal Antibodies
Monoclonal Antibodies
Recombinant Monoclonal Antibodies
Secondary Antibodies
Recombinant Proteins
Protein on Demand™ Custom Recombinant Proteins
Peptide
Western Blot Reagents
Sample Preparation
Services
High Throughput Antibody Characterization Services
Custom Antibodies
Custom Recombinant Protein Production
ELISA Development
ISO 13485/CMO Services
Conjugation Services
Aviva Systems Biology - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"
MSDS
온라인 견적
