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폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.
제품 설명
Prealbumin ELISA Kit (Mouse)
제품 번호
OKIA00111 (Formerly GWB-374Z37)
제품 특징
Product Info
| Predicted Species Reactivity | Monkey | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Product Format | In Solution | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||
| ELISA Kit Duration | 60 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Prealbumin ELISA Kit (Mouse) (OKIA00111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well. | ||||||||||||||||
| ELISA Kit Range | 15.6 ng/ml - 500 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of mice. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Mouse Prealbumin antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-MOUSE PREALBUMIN ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse PRE. 7. MOUSE PREALBUMIN STANDARDS One vial containing a lyophilized Mouse Prealbumin calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 3.967 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Mouse | ||||||||||||||||
| Publications | Jonathan D Finn, Amy Rhoden Smith, Mihir C Patel, Lucinda Shaw, Madeleine R Youniss, Jane van Heteren, Tanner Dirstine, Corey Ciullo, Reynald Lescarbeau, Jessica Seitzer, Ruchi R Shah, Aalok Shah, Dandan Ling, Jacqueline Growe, Melissa Pink, Ellen Rohde, Kristy M Wood, William E Salomon, William F Harrington, Christian Dombrowski, Walter R Strapps, Yong Chang, David V Morrissey. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing.. Cell Rep. 22, 2227-2235 (2018). 29490262 Charleen Hunt, Suzanne A Hartford, Derek White, Evangelos Pefanis, Timothy Hanna, Clarissa Herman, Jarrell Wiley, Heather Brown, Qi Su, Yurong Xin, Dennis Voronin, Hien Nguyen, Judith Altarejos, Keith Crosby, Jeffery Haines, Sarah Cancelarich, Meghan Drummond, Sven Moller-Tank, Ryan Malpass, Jacqueline Buckley, Maria Del Pilar Molina-Portela, Gustavo Droguett, David Frendewey, Eric Chiao, Brian Zambrowicz, Guochun Gong. Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice.. Nat Commun. 12, 2770 (2021). 33986266 Jenny A Greig, Camilo Breton, Scott N Ashley, Kelly M Martins, Cassandra Gorsuch, Joanna K Chorazeczewski, Thomas Furmanak, Melanie K Smith, Yanqing Zhu, Peter Bell, Wendy Shoop, Hui Li, Jeff Smith, Ginger Tomberlin, Peter Clark, Thomas W Mitchell, Elizabeth L Buza, Hanying Yan, Derek Jantz, James M Wilson. Treating Transthyretin Amyloidosis via Adeno-Associated Virus Vector Delivery of Meganucleases.. Hum Gene Ther. 33, 1174-1186 (2022). 36375122 | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Target Info
| Gene Symbol | TTR |
|---|---|
| Gene Full Name | Transthyretin |
| Alias Symbols | prea, D17860, AA408768, AI787086, prealbumin |
| NCBI Gene Id | 22139 |
| Protein Name | Transthyretin |
| Description of Target | This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease. |
| Uniprot ID | P07309 |
Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.
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