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[Aviva] Prealbumin ELISA Kit (Rat)

Cat-No. OKIA00159 (Formerly GWB-374PRE)


Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록 

폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.




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Prealbumin ELISA Kit (Rat)

  



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OKIA00159 (Formerly GWB-374PRE)



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Product Info

Product Info
Predicted Species ReactivityRat
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration60 Minutes
ELISA Kit PrincipleAviva Systems Biology Prealbumin ELISA Kit (Rat) (OKIA00159) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well.
ELISA Kit Range15.625 ng/ml - 500 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rat Prealbumin (Transthyretin) Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat Prealbumin (Transthyretin) Calibrator1
Additional InformationIntended Use: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of rat.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Prealbumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT PREALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat PRE.
7. RAT PREALBUMIN STANDARDS
One vial containing a lyophilized Rat Prealbumin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity4.508 ng/ml
Predicted Homology Based on Immunogen SequenceRat
Publications

Takuya Adachi, Yukari Yoshida, Kei Shibuya, Ken Sato, Yuichi Yamazaki, Satoru Kakizaki, Toshio Uraoka, Akihisa Takahashi. Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation.. J Radiat Res. 64, 693-701 (2023). 37427542

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Assay InfoAssay Methodology: Quantitative Sandwich ELISA


Target Info

Gene SymbolTTR
Gene Full NameTransthyretin
Alias SymbolsTT, Lr1, Tbpa
NCBI Gene Id24856
Protein NameTransthyretin
Description of Targetbinds thyroxine (T4) and 3,5,3' triiodothyronine (T3); plays a role in thyroid hormone transport in serum.
Uniprot IDP02767




Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.




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