Aviva Systems Biology는 기초 연구부터 전임상, 신약 개발, 진단 워크플로우 전반에서 사용할 수 있도록
폴리클로널·모노클로날 항체, 재조합 단백질, ELISA 키트, 그리고 맞춤형 단백질·항체 개발 서비스를 제공합니다.
제품 설명
Prealbumin ELISA Kit (Rat)
제품 번호
OKIA00159 (Formerly GWB-374PRE)
제품 특징
Product Info
Predicted Species Reactivity | Rat | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Product Format | In Solution | ||||||||||||||||
Application | ELISA | ||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||
ELISA Kit Duration | 60 Minutes | ||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Prealbumin ELISA Kit (Rat) (OKIA00159) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well. | ||||||||||||||||
ELISA Kit Range | 15.625 ng/ml - 500 ng/ml | ||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||
Additional Information | Intended Use: The Prealbumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Prealbumin in biological samples of rat. | ||||||||||||||||
:: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Rat Prealbumin antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-RAT PREALBUMIN ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat PRE. 7. RAT PREALBUMIN STANDARDS One vial containing a lyophilized Rat Prealbumin calibrator. | ||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
Sensitivity | 4.508 ng/ml | ||||||||||||||||
Predicted Homology Based on Immunogen Sequence | Rat | ||||||||||||||||
Publications | Takuya Adachi, Yukari Yoshida, Kei Shibuya, Ken Sato, Yuichi Yamazaki, Satoru Kakizaki, Toshio Uraoka, Akihisa Takahashi. Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation.. J Radiat Res. 64, 693-701 (2023). 37427542 | ||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Target Info
Gene Symbol | TTR |
---|---|
Gene Full Name | Transthyretin |
Alias Symbols | TT, Lr1, Tbpa |
NCBI Gene Id | 24856 |
Protein Name | Transthyretin |
Description of Target | binds thyroxine (T4) and 3,5,3' triiodothyronine (T3); plays a role in thyroid hormone transport in serum. |
Uniprot ID | P02767 |
Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.
Aviva Systems Biology의 모든 제품을 만나 보세요!
Products
Recombinant Monoclonal Antibodies
Protein on Demand™ Custom Recombinant Proteins
Services
High Throughput Antibody Characterization Services
Custom Recombinant Protein Production
Aviva Systems Biology - Official Distributor in South Korea "Morebio" 한국 공식 대리점 "모아바이오"