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제품 설명
Cas9 Nuclease (10mg/mL)
제품 번호
14701ES
제품 설명
Cas9 Nuclease is derived from the wild-type Streptococcus pyogenes and is an RNA-guided endonuclease that specifically cleaves double-stranded DNA (it can also cleave single-stranded DNA or RNA in the presence of a DNA PAM). The Cas9 cleavage site is located within the target sequence, three base pairs away from the PAM (NGG) region. Cas9 Nuclease has undergone codon optimization and design with a nuclear localization signal (NLS), and is expressed through recombinant expression in Escherichia coli. It exhibits high editing efficiency and can be used for gene modification in cells (such as hematopoietic stem cells, T cells, etc.), as well as for molecular diagnostics and pathogen detection.
Optimized nuclear localization signal: The optimized nuclear localization signal (NLS) enhances editing efficiency.
High editing efficiency: Consistently high editing efficiency both in vitro and in vivo.
High purity: The purity is greater than or equal to 95%.
High concentration: It is applicable under standard editing conditions and is also suitable for optimizing editing conditions in more challenging scenarios, such as in primary or embryonic cells, through microinjection or when conducting high-throughput screening of multiple gRNA sequences.
CRISPR/Cas gene editing
Diagnostic and detection based on the CRISPR/Cas system
Source | The Cas9 gene from Streptococcus pyogenes is expressed through recombinant expression in Escherichia coli |
Molecular weight | 163 KDa |
Concentration | 10 mg/mL |
Purity | ≥95% |
Tag | His |
Endotoxin | ≤10 EU/mg |
This product should be stored at -25~-15℃ for 1 years.
Components No. | Name | 14701ES60 | 14701ES76 | 14701ES03 |
14701-A | Cas9 Nuclease（10 mg/mL） | 10 μL | 50 μL | 100 μL |
14701-B | 10×Cas9 Nuclease Reaction Buffer | 500 μL | 1 mL | 1 mL |
Figure 1. Results of Cas9 nuclease in vitro cleavage activity and gene knockout efficiency
Q: When using 14701ES, how should one design single-guide RNA (sgRNA)?
A: This product is derived from the wild-type Streptococcus pyogenes. The single-strand RNA (sgRNA) backbone can be used.
5’-NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAUGCUGAAAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUG -3’
"N" represents a 20-nucleotide sequence that specifically binds to the DNA target sequence.
We recommend purchasing the sgRNA synthesis kit with item number 11355ES from Yisheng. It has a high yield. Just design based on the target region, and the purified sgRNA product can be used in combination with 14701.
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