AFG Scientific은 2만 가지 이상 다양한 종류의 ELISA KITS와 Antibodies 제품들을 

High Quality 등급으로 전문 생산하며 합리적인 가격을 제공하는 미국의 Biological 제조사입니다.

제품 설명

Human HMGCR (3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) Elisa Kit

제품 번호

EK244645

제품 특징

시약 준비 과정

Bring all reagents to room temperature (18–25°C) before use. If the kit will not be fully utilized in one assay, please take out only the necessary strips and reagents for the current experiment and store the remaining strips and reagents under the required conditions. If the kit will not be used in one session, only take out the strips and reagents needed for the current experiment, and save the remaining strips and reagents as specified.

Dilute the 25× Wash Buffer to 1× Wash Buffer with double-distilled water.

Centrifuge the standard at the specified speed for 1 minute. Reconstitute the standard with the designated volume of Standard Diluent Buffer, allowing it to sit at room temperature for approximately 10 minutes. Gently shake the solution to avoid foaming.

Prepare seven tubes, each containing a specific volume of Standard Diluent Buffer, and use the diluted standard to create a double dilution series. Mix each tube thoroughly before proceeding to the next transfer by pipetting the solution up and down several times. Establish a series of diluted standards across the tubes, ending with a final tube that contains only Standard Diluent, which will serve as the blank. To ensure the validity of your experimental results, always use a new standard solution for each experiment. When performing the dilutions, change the pipette tip for each new dilution to prevent cross-contamination. Remember that the last tube is designated as the blank; do not transfer any solution into it from the preceding tube.

Tube	6 (Stock)	5	4	3	2	1	0 (Blank)
ng/ml	100	50	25	12.5	6.25	3.13	0

Preparation of 1× Biotinylated-Conjugate and 1× Streptavidin-HRP Working Solution:

1. Briefly spin or centrifuge the stock solutions of Biotinylated-Conjugate and Streptavidin-HRP to ensure they are well mixed.
2. Dilute each solution to a working concentration of 100-fold:
   • For the Biotinylated-Conjugate, use the Biotinylated-Conjugate Diluent.
   • For the Streptavidin-HRP, use the HRP Diluent.
3. For example, to prepare the Streptavidin-HRP solution, mix 10 µL of Streptavidin-HRP with 990 µL of HRP Diluent.

TMB Substrate Solution — Aspirate the required dosage of the solution with sterile tips, and do not pour the residual solution back into the vial.

AFG Scientifics에서 다양한 제품을 찾아보세요! 

• Antibodies
• Chemicals
• Elisa Kits
• Chromatography

AFG Scientific - Official Distributor in South Korea "Morebio" 한국 독점 대리점 "모아바이오"