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Biotechrabbit

[Biotechrabbit] dNTP Sets

Cat-No. BR0600601


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


dNTP Sets



제품 번호


BR0600601 - 4 × 250 µl

BR0600602 - 4 × 1ml


제품 특징


Features

  • Exceptional quality dNTPs of >99% purity confirmed by HPLC
  • Free from DNA and PCR inhibitors
  • Consistent PCR results due to outstanding dNTPs stability

Applications

  • Standard or hot-start PCR
  • Long-range and high-fidelity PCR
  • cDNA synthesis and RT-PCR
  • qPCR
  • Sequencing
  • DNA labeling



Description


biotechrabbit™ deoxynucleotide triphosphates are first-choice nucleotides for all PCR applications, including the most demanding, such as amplification of long targets (up to 40 kb), GC-rich templates, qPCR, cDNA synthesis, high-fidelity PCR, DNA labeling and sequencing.

Advanced production technology ensures that deoxyribonucleotide triphosphates have >99% purity and outstanding stability, ensuring excellent performance and consistent, reliable results.

For the maximum flexibility, nucleotides are available in sets and mixes of common concentrations.



Other Information


Component

Composition

all dNTP Sets

Aqueous solution (pH 7.0) containing
2'-deoxy-nucleotide 5'-triphosphate sodium salts

 

STORAGE

−20°C (until expiry date – see product label)

 

Quality Control
Functional assay

Functionality tested in PCR.

Purity test

The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test

The absence of human and E. coli DNA is confirmed by qPCR.




Protocols

Prevention of PCR contamination

When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA.

  • Use separate clean areas for preparation of samples and reaction mixtures and for cycling.
  • Wear fresh gloves. Use sterile tubes and pipette tips with aerosol filters for PCR setup.
  • Use only water and reagents that are free of DNA and nucleases.
  • With every PCR setup, perform a contamination control reaction that does not include template DNA.
Typical use of dNTPs
  • 0.2 mM of each nucleotide is the recommended final dNTP concentration in common PCR applications.
  • It is essential to have equal concentrations of all four dNTPs in the PCR mixture.
  • Every time before the use thaw and mix well each dNTP solution, especially highly concentrated ones.
  • To prepare on your own 1 ml dNTP mixtures of common 2 mM, 2.5 mM, 10 mM or 25 mM concentrations, follow the guidelines below:

1 ml Mix

100 mM dATP

100 mM dCTP

100 mM dGTP

100 mM dTTP

Water

2 mM dNTP Mix:

20 µl

20 µl

20 µl

20 µl

920 µl

2.5 mM dNTP Mix:

25 µl

25 µl

25 µl

25 µl

900 µl

10 mM dNTP Mix:

100 µl

100 µl

100 µl

100 µl

600 µl

25 mM dNTP Mix:

250 µl

250 µl

250 µl

250 µl

-

 

  • Use the ready dNTP mixes in PCR (at 0.2 mM final concentration each) according to the guidelines below:

Volumes of dNTP mixes for the indicated PCR volume

dNTP Mix concentration

Used for PCR as

50 µl PCR

25 µl PCR

20 µl PCR

2 mM

10×

5 µl

2.5 µl

2 µl

2.5 mM

12.5×

4 µl

2 µl

1.6 µl

10 mM

50×

1 µl

0.5 µl

0.4 µl

25 mM

125×

0.4 µl

0.2 µl

0.16 µl








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