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제품상세페이지

Biotechrabbit

[Biotechrabbit] dNTP Mixes

Cat-No. BR0600502


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.




제품 설명


dNTP Mixes




제품 번호


BR0600502 - 1ml / 1 ml dNTP Mix (25 mM each)

BR0600503 - 5ml / 5 x 1 ml dNTP Mix (25 mM each)

BR0600202 - 1ml / 1 ml dNTP Mix (10 mM each)

BR0600204 - 5ml / 5 x 1 ml dNTP Mix (10 mM each)

BR0601101 - 1ml / 1 ml dNTP/dUTP Mix (10 mM A/C/G, 30 mM U)

BR0601102 - 5ml / 5 x 1 ml dNTP/dUTP Mix (10 mM A/C/G, 30 mM U)



제품 특징


Features

  • Exceptional quality dNTPs of >99% purity confirmed by HPLC
  • Free from DNA and PCR inhibitors
  • Consistent PCR results due to outstanding dNTPs stability

Applications

  • Standard or hot-start PCR
  • Long-range and high-fidelity PCR
  • cDNA synthesis and RT-PCR
  • qPCR
  • Sequencing
  • DNA labeling



Description


biotechrabbit™ deoxynucleotide triphosphates are first-choice nucleotides for all PCR applications, including the most demanding, such as amplification of long targets (up to 40 kb), GC-rich templates, qPCR, cDNA synthesis, high-fidelity PCR, DNA labeling and sequencing.

Advanced production technology ensures that deoxyribonucleotide triphosphates have >99% purity and outstanding stability, ensuring excellent performance and consistent, reliable results.

For the maximum flexibility, nucleotides are available in sets and mixes of common concentrations.



Other Information


Component

Composition

all dNTP Mixes

Aqueous solution (pH 7.0) containing
2'-deoxy-nucleotide 5'-triphosphate sodium salts

 

STORAGE

−20°C (until expiry date – see product label)

 

Quality Control
Functional assay

Functionality tested in PCR.

Purity test

The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test

The absence of human and E. coli DNA is confirmed by qPCR.




Protocols

Prevention of PCR contamination

When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA.

  • Use separate clean areas for preparation of samples and reaction mixtures and for cycling.
  • Wear fresh gloves. Use sterile tubes and pipette tips with aerosol filters for PCR setup.
  • Use only water and reagents that are free of DNA and nucleases.
  • With every PCR setup, perform a contamination control reaction that does not include template DNA.
Typical use of dNTPs
  • 0.2 mM of each nucleotide is the recommended final dNTP concentration in common PCR applications.
  • Every time before the use thaw and mix well each dNTP solution, especially highly concentrated ones.
  • Use the ready dNTP mixes in PCR (at 0.2 mM final concentration each


Documents

Product manual 25 mM dNTP Mix

Product manual 10 mM dNTP Mix

Product manual 10 mM dN/UTP Mix




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