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Biotechrabbit

[Biotechrabbit] GenUP™ PCR Cleanup Kit

Cat-No. BR0700301


Biotechrabbit은 바이오의약품 및 생명과학 연구를 위한 고급 실험 재료와 서비스를 제공하는 회사입니다.



제품 설명


GenUP™ PCR Cleanup Kit



제품 번호


BR0700301 - 10 preps

BR0700302 - 50 preps

BR0700303 - 250 preps


제품 특징


Features

  • Fast and convenient PCR cleanup procedure in only 3 min
  • Just bind and elute, no need for washing steps
  • High-purity DNA recovery for all demanding applications

Applications

  • Fast purification of products from PCR amplification reactions



Description


biotechrabbit™ GenUP PCR Cleanup Kit has been developed for quick and easy cleanup or concentration of PCR fragments from reaction mixtures. High-yield PCR product with excellent quality is purified from the PCR mixtures in a simple two-step procedure. DNA is bound to a Mini Filter using a novel buffer and eluted. The need for a washing step has been eliminated, reducing hands-on-time. The procedure takes approximately 3 min, compared to 8 min required to other kits. The purified DNA is ready for use in all demanding molecular biology applications, including restriction digestion, ligation and sequencing.



Other Information


STARTING MATERIAL

Amplification reaction mixtures (up to 50 µl)

EXTRACTION TIME

Approximately 3 min

BINDING CAPACITY

> 20 µg DNA

DNA SIZE

60 bp – 30 kb

RECOVERY RATE

60–95%, depending on length of the PCR fragment




Protocols

STEPS

  • Transfer the PCR sample to a centrifugation tube, add Buffer BINDING BP and mix well.
  • Transfer the sample to a Mini Filter (green) placed in a Collection Tube and centrifuge.
  • Add Buffer ELUTION, incubate and centrifuge.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).





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